Rat C-X-C motif chemokine 9(CXCL9) ELISA kit

产品详情

产品描述：

大鼠趋化因子9(CXCL9)酶联免疫试剂盒（CSB-EL006252RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CXCL9含量。CXCL9是重要的靶点。它属趋化因子家族，在免疫调节中起关键作用。其研究机制主要聚焦于对免疫细胞的趋化作用，招募特定免疫细胞到炎症或肿瘤部位，影响免疫应答过程，在肿瘤免疫、自身免疫病等领域研究价值高。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外实验环境下CXCL9表达水平的动态监测，如炎症模型构建、免疫相关分子机制研究、药物干预效果评估等科研场景，为探索CXCL9在免疫调控网络中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

CMK, Humig, MIG, SCYB9, crg-10, monokine induced by gamma interferon|small inducible cytokine B9
缩写：

CXCL9
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CXCL9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	102
	Range %	97-107
1:4	Average %	92
	Range %	88-96
1:8	Average %	105
	Range %	99-110

回收率：

The recovery of rat CXCL9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	81-96
EDTA plasma (n=4)	98	92104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.