Rat C-C motif chemokine 6 (CCL6) ELISA kit

产品详情

产品描述：

大鼠C-C趋化因子6(CCL6)酶联免疫试剂盒（CSB-EL027415RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CCL6含量。CCL6属于趋化因子，能趋化特定免疫细胞到炎症部位。其研究机制主要围绕它与受体相互作用，调节免疫细胞迁移和活化。深入研究CCL6对揭示免疫调节机制有意义，也有助于探索相关疾病如炎症性疾病、肿瘤等的治疗靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，可为科研人员研究炎症性疾病发生机制、免疫细胞趋化行为调控、感染病理模型中的细胞因子网络变化等课题提供可靠工具，尤其适用于动物实验模型中趋化因子动态监测、药物干预效果评估等基础研究场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Ccl6C-C motif chemokine 6 ELISA Kit; Small-inducible cytokine A6 ELISA Kit
缩写：

CCL6
Uniprot No.：

Q68FP3
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CCL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	87-98
1:2	Average %	98
	Range %	91-104
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	92-103

回收率：

The recovery of rat CCL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	98	93-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

基因功能参考文献：
1. The mouse C10 analog gene in the rat, and named it as ccl6, was identified and cloned. PMID: 23212328
亚细胞定位：

Secreted.
蛋白家族：

Intercrine beta (chemokine CC) family
数据库链接：

KEGG: rno:287910

STRING: 10116.ENSRNOP00000048296

UniGene: Rn.7857