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Rat Apoptosis regulator BAX(BAX) ELISA kit

  • 中文名称:
    大鼠细胞凋亡调节因子BAX(BAX)酶联免疫试剂盒
  • 货号:
    CSB-EL002573RA
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO大鼠细胞凋亡调节因子BAX(BAX)酶联免疫试剂盒(货号:CSB-EL002573RA),基于双抗体夹心法定量原理开发,适用于血清、血浆、组织匀浆及细胞裂解液样本中BAX蛋白含量的精准检测。BAX作为Bcl-2家族关键成员,通过调控线粒体膜通透性介导细胞凋亡信号通路,在发育调控、肿瘤发生及神经退行性疾病等研究中具有重要价值。试剂盒检测范围为62.5-4000 pg/mL,最低检测灵敏度达62.5 pg/mL,预包被高特异性抗体确保检测的稳定性与准确性。实验流程包含标准品梯度稀释、样本孵育、生物素化抗体结合及TMB显色步骤,显色时间约15分钟,通过酶标仪测定450 nm吸光度值完成定量分析。该试剂盒配套提供预包被96孔板、标准品、检测抗体及终止液等完整组分,适用于科研场景中细胞凋亡机制研究、药物诱导的凋亡效应评估或疾病模型(如缺血再灌注损伤、癌症)中BAX表达水平的动态监测,为探索凋亡相关信号通路提供可靠工具。
  • 别名:
    Bax; Apoptosis regulator BAX
  • 缩写:
  • Uniprot No.:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围:
    62.5 pg/mL-4000 pg/mL
  • 灵敏度:
    15.6 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cell Biology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat BAX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 89  
    Range % 85-92  
    1:2 Average % 96  
    Range % 91-105  
    1:4 Average % 90  
    Range % 87-99  
    1:8 Average % 93  
    Range % 82-98  
  • 回收率:
    The recovery of rat BAX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-97  
    EDTA plasma (n=4) 97 93-105  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    4000 2.091 1.995 2.043 1.844  
    2000 1.751 1.776 1.764 1.565  
    1000 1.368 1.432 1.400 1.201  
    500 0.985 0.972 0.979 0.780  
    250 0.535 0.544 0.540 0.341  
    125 0.348 0.352 0.350 0.151  
    62.5 0.244 0.237 0.241 0.042  
    0 0.201 0.197 0.199    
  • 数据处理:
  • 货期:
    3-5 working days

引用文献

产品评价

靶点详情

  • 最新研究进展:
    BAX是一种参与调控细胞凋亡的蛋白质,属于Bcl-2家族。最近的研究表明,BAX不仅在细胞凋亡中发挥重要作用,而且在多种疾病的发生中也具有重要作用。例如,BAX在癌症、神经系统疾病、肝脏疾病等的发生中都发挥了重要作用。此外,一些研究还发现BAX在代谢调节、免疫系统调节等方面也具有重要作用。
  • 功能:
    Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
  • 亚细胞定位:
    [Isoform Alpha]: Mitochondrion outer membrane; Single-pass membrane protein. Cytoplasm.; [Isoform Beta]: Cytoplasm.; [Isoform Gamma]: Cytoplasm.
  • 蛋白家族:
    Bcl-2 family
  • 组织特异性:
    Expressed in a wide variety of tissues, with highest levels in the testis and ovary.
  • 数据库链接: