Rat Apolipoprotein C-III(APOC3) ELISA kit

产品详情

产品描述：

大鼠载脂蛋白C-III(APOC3)酶联免疫试剂盒（CSB-EL001933RA）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOC3含量。APOC3即载脂蛋白C3，它可抑制脂蛋白脂肪酶活性、阻碍肝脏对甘油三酯的摄取，与血浆甘油三酯水平密切相关。降低其水平可减少甘油三酯，目前围绕它开发降血脂药物是研究热点，有望为心血管疾病防治提供新策略。试剂盒检测范围为31.25 ng/mL-2000 ng/mL，适用于脂代谢机制研究、药物干预评价及疾病动物模型（如高脂饮食诱导模型或基因修饰动物）的病理分析，为心血管疾病、肥胖症等领域的科研实验提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Apoc3 ELISA Kit; Apolipoprotein C-III ELISA Kit; Apo-CIII ELISA Kit; ApoC-III ELISA Kit; Apolipoprotein C3 ELISA Kit
缩写：

APOC3
Uniprot No.：

P06759
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 ng/mL-2000 ng/mL
灵敏度：

7.8 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat APOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	88-96
1:200	Average %	95
	Range %	91-99
1:400	Average %	93
	Range %	90-97
1:800	Average %	95
	Range %	89-99

回收率：

The recovery of rat APOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-93
EDTA plasma (n=4)	87	84-90

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors. Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners.
亚细胞定位：

Secreted.
蛋白家族：

Apolipoprotein C3 family
组织特异性：

Synthesized predominantly in liver and to a lesser degree in intestine.
数据库链接：

STRING: 10116.ENSRNOP00000067045

UniGene: Rn.129547