Rat Antithrombin III(AT III)ELISA Kit

产品详情

产品描述：

CUSABIO大鼠抗凝血酶III（AT III）酶联免疫检测试剂盒（货号：CSB-E13885r），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的AT III蛋白水平。抗凝血酶III是凝血级联反应的核心调节因子，通过与凝血酶及活化凝血因子结合发挥天然抗凝作用，在维持血液稳态及血栓性疾病研究中具有重要价值。该试剂盒检测范围为39-2500 ng/mL，具有高灵敏度和宽线性范围，适用于各类科研实验中AT III水平的精确分析。实验操作采用预包被特异性抗体的96孔板，通过标准品与样本同步孵育、酶联反应及显色测定，可实现多样本的高通量检测。其优化的检测体系可兼容常规生物样本类型，尤其适用于心血管疾病模型、药物抗凝机制研究、肝脏功能评估等科研场景，为探索凝血-抗凝系统调控及病理生理机制提供可靠工具。试剂盒配套提供标准品、检测抗体及全套实验缓冲液，操作流程规范，结果稳定可重复。
缩写：

AT III
Uniprot No.：

Q5M7T5
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

39 ng/mL-2500 ng/mL
灵敏度：

9.75 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat AT III in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	104
	Range %	100-112
1:400	Average %	84
	Range %	80-88
1:800	Average %	90
	Range %	85-94
1:1600	Average %	97
	Range %	92-101

回收率：

The recovery of rat AT III spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	93	89-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.