Rat Anti-Cyclic Citrullinated Peptide Antibody(Anti-CCP-antibody)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测大鼠血清或血浆中抗环胍氨酸肽抗体（Anti-CCP-antibody）的酶联免疫试剂盒，采用双抗体夹心法定量分析样本中靶标含量，为类风湿性关节炎等自身免疫性疾病机制研究提供高效工具。Anti-CCP抗体是类风湿性关节炎特异性自身抗体，其检测对疾病早期诊断及病程监测具有重要科研价值。该试剂盒线性检测范围覆盖3.12-200 IU/mL，灵敏度达3.12 IU/mL，适用于体外实验中定量评估血清、血浆等生物样本中抗体水平。试剂盒包含预包被抗体的酶标板、标准品、检测抗体及显色底物体系，操作流程优化后可在4小时内完成检测。科研人员可通过该产品开展疾病模型中的抗体动态变化研究、药物干预效果评价或探索CCP抗体与关节炎症的相关性，为免疫病理机制解析及新型治疗策略开发提供数据支持。所有组分均经过严格验证，确保批次间稳定性和实验重复性。
缩写：

CCP Ab
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma
检测范围：

3.12 IU/mL--200 IU/mL
灵敏度：

0.78 IU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat anti-CCP-antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	85-105
1:2	Average %	97
	Range %	84-105
1:4	Average %	96
	Range %	92-100
1:8	Average %	95
	Range %	88-105

回收率：

The recovery of rat anti-CCP-antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-96
EDTA plasma (n=4)	94	85-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.