Rat Angiopoietin-related protein 4(ANGPTL4) ELISA kit

Rat Angiopoietin-related protein 4(ANGPTL4) ELISA kit

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中文名称：

大鼠血管生成素相关蛋白4(ANGPTL4)酶联免疫试剂盒
货号：

CSB-EL001712RA
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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大鼠血管生成素相关蛋白4(ANGPTL4)酶联免疫试剂盒（CSB-EL001712RA）检测血清、血浆、组织培养上清液、组织匀浆、支气管肺泡灌洗液、尿液样本中的ANGPTL4含量。ANGPTL4是一个重要靶点。它属于血管生成素样蛋白家族，在脂质代谢、血管生成等生理过程中发挥作用。研究发现，它可抑制脂蛋白脂肪酶活性影响甘油三酯代谢。同时其在肿瘤血管生成等病理机制中也有参与，为相关疾病治疗提供了潜在方向。试剂盒检测范围为7.8 pg/mL-500 pg/mL，本产品适用于肥胖、糖尿病、动脉粥样硬化等代谢相关疾病的机制研究，或炎症模型、血管新生实验的分子水平分析，为体外实验提供高灵敏度的定量检测方案本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Angptl4Angiopoietin-related protein 4 ELISA kit; Angiopoietin-like protein 4 ELISA kit; Hepatic fibrinogen/angiopoietin-related protein ELISA kit; HFARP) [Cleaved into: ANGPTL4 N-terminal chain; ANGPTL4 C-terminal chain] ELISA kit
缩写：

ANGPTL4
Uniprot No.：

Q6TMA8
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates, bronchoalveolar lavage fluid, urine
检测范围：

7.8 pg/mL-500 pg/mL
灵敏度：

1.95 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ANGPTL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	87
Range %	83-102
1:400	Average %	86
Range %	81-90
1:800	Average %	92
Range %	87-98
1:1600	Average %	102
Range %	99-105

回收率：

The recovery of rat ANGPTL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	89	85-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.634	2.687	2.661	2.561
250	2.226	2.268	2.247	2.147
125	1.568	1.534	1.551	1.451
62.5	0.968	0.943	0.956	0.856
31.25	0.527	0.568	0.548	0.448
15.62	0.398	0.377	0.388	0.288
7.8	0.274	0.289	0.282	0.182
0	0.099	0.101	0.100

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. May also play a role in regulating glucose homeostasis and insulin sensitivity. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro). Depending on context, may modulate tumor-related angiogenesis.; Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism. Has higher activity in LPL inactivation than the uncleaved protein.
基因功能参考文献：
1. this study represents the first investigation to demonstrate that Angptl4 can predict podocyte injury at earlier stages in minimal change disease PMID: 26352670
2. GR and FoxO1 are required for Angptl4 transcription activation, and that FoxO1 negatively mediates the suppressive effect of insulin PMID: 24565756
3. the overexpression of Angptl4 exerts protective, anti-inflammatory and anti-angiogenic effects. It re-presents a novel therapeutic target gene for the treatment of acute lung injury induced by LPS. PMID: 23783408
4. Data suggest that endothelial lipoprotein lipase (LPL) levels in the heart are altered to maintain FA flux and may exploit angiopoietin-like protein-4 (ANGPTL4). PMID: 22226882
5. Angptl4 is a novel methylation-silenced gene both in rat and human mammary carcinomas. PMID: 21489049
6. Angptl-4 is a fasting-induced controller of lipoprotein lipase in adipose tissue, acting extracellularly on the native conformation in an unusual fashion, like an unfolding molecular chaperone PMID: 17088546
7. ANGPTL4 is a direct GR target that participates in glucocorticoid-regulated triglyceride metabolism. PMID: 19628874
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亚细胞定位：

Secreted. Secreted, extracellular space, extracellular matrix.
数据库链接：

KEGG: rno:362850

STRING: 10116.ENSRNOP00000010031

UniGene: Rn.119611

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.