Rat Alpha-fetoprotein,AFP ELISA Kit

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中文名称：

大鼠甲种胎儿球蛋白/甲胎蛋白(AFP)酶联免疫试剂盒
货号：

CSB-E08281r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠甲种胎儿球蛋白/甲胎蛋白（AFP）酶联免疫检测试剂盒（货号：CSB-E08281r），采用双抗体夹心法定量检测样本中AFP含量。AFP是一种在胚胎发育阶段由肝脏和卵黄囊合成的功能性糖蛋白，正常成年个体中表达水平较低，但在肝癌或部分生殖细胞肿瘤模型中可能异常升高，是研究肝脏再生、胚胎发育及肿瘤发生的重要生物标志物。本试剂盒检测灵敏度高，线性范围为15.6 pg/mL至1000 pg/mL，适用于分析血清、血浆、细胞培养上清液及组织匀浆等多种样本类型。实验通过预包被特异性捕获抗体与样本中AFP结合，再经检测抗体和酶标记物形成复合物，最终通过显色反应实现定量分析。该产品可广泛应用于肝癌动物模型研究、干细胞分化监测、组织再生机制探索及肿瘤相关信号通路分析等科研领域。操作时建议根据样本类型调整预处理条件，如高脂血清需离心去浊，组织样本需充分匀浆并离心取上清液，以确保检测结果准确性。本试剂盒仅供科学研究使用，所有组分均经过严格质控。
别名：

AfpAlpha-fetoprotein ELISA Kit; Alpha-1-fetoprotein ELISA Kit; Alpha-fetoglobulin ELISA Kit
缩写：

AFP
Uniprot No.：

P02773
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	85
	Range %	81-90
1:40	Average %	89
	Range %	82-94
1:80	Average %	101
	Range %	96-108
1:160	Average %	94
	Range %	89-99

回收率：

The recovery of rat AFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-94
EDTA plasma (n=4)	97	93-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.701	2.631	2.666	2.478
500	2.239	2.212	2.226	2.038
250	1.710	1.663	1.687	1.499
125	1.097	1.174	1.136	0.948
62.5	0.651	0.695	0.673	0.485
31.2	0.427	0.459	0.443	0.255
15.6	0.337	0.317	0.327	0.139
0	0.192	0.184	0.188