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  4. Rat Alanine aminotransferase(ALT) ELISA Kit

Rat Alanine aminotransferase(ALT) ELISA Kit

文献引用 (16) 说明书下载
  • 中文名称：
    大鼠丙氨酸氨基转移酶(ALT)酶联免疫试剂盒
  • 货号：
    CSB-E13024r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO专为大鼠样本设计的丙氨酸氨基转移酶（ALT）定量检测试剂盒（货号：CSB-E13024r），采用双抗体夹心法原理，可灵敏检测3.12-200 mIU/mL浓度范围的ALT活性。ALT作为肝脏功能关键指标，其水平变化与肝细胞损伤密切相关，广泛应用于科研领域对肝脏代谢及损伤机制的研究。试剂盒兼容多种样本类型，包括血清、血浆、细胞培养上清及经标准方法制备的组织匀浆，为实验设计提供灵活选择。检测体系通过预包被高特异性抗体实现精准捕获目标蛋白，结合优化反应条件确保数据可靠性。该产品适用于实验室中肝毒性评估、药物代谢研究、疾病动物模型建立等场景，例如可通过检测不同处理条件下肝组织匀浆或血清中的ALT水平变化，评估化学物质、药物或病理状态对肝脏功能的影响。试剂盒提供完整的标准品及预混试剂，操作流程标准化，支持在常规实验室条件下开展高效、稳定的ALT活性定量分析。
  • 别名：
    Gpt ELISA Kit; Aat1 ELISA Kit; Gpt1 ELISA Kit; Alanine aminotransferase 1 ELISA Kit; ALT1 ELISA Kit; EC 2.6.1.2 ELISA Kit; Glutamate pyruvate transaminase 1 ELISA Kit; GPT 1 ELISA Kit; Glutamic--alanine transaminase 1 ELISA Kit; Glutamic--pyruvic transaminase 1 ELISA Kit
  • 缩写：
    GPT
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    3.12 mIU/mL-200 mIU/mL
  • 灵敏度：
    0.78 mIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat ALT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %98
    Range %91-105
    1:4Average %100
    Range %92-110
    1:8Average %94
    Range %86-98
  • 回收率：
    The recovery of rat ALT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9690-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    2002.814 2.911 2.863 2.778
    1002.466 2.355 2.411 2.326
    501.659 1.564 1.612 1.527
    250.853 0.812 0.833 0.748
    12.50.469 0.442 0.456 0.371
    6.250.243 0.226 0.235 0.150
    3.120.149 0.141 0.145 0.060
    00.088 0.082 0.085 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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相关问答

■ 常见问题解答
Q:

Kindly address the below queries:
1. The standard ALT enzyme provided in the kit is from which source ?
2. The Standard ALT enzyme given is active or inactive ?
3. Since, we cannot collect more than 150 µL of blood from mice, we can use only 50 µL serum for ELISA. Will it fall within the detectable range ?

A:
We are glad to inform you the following details:
CSB-E13024r,Rat Alanine aminotransferase(ALT) ELISA Kit
Standard:293 cell-derived recombinant protein
Sorry we did not do the activity analysis on the standard.
As to the sample loading volume, we suggest you refer to the manual/datasheet of these 2 kits. If the sample volume is too little, the target might not be recognized during the assay.
Q:

CSB-E13024r
1. In what units, the final data will be represented ? i.e in enzyme activity (IU/L) or
enzyme levels (ng/mg of protein).
2. The reference articles (Article attached) given in the website has mentioned the result
in U/I. What is this unit and how this unit is derived ?
3. Kindly mention the species in which the primary antibody (used in this kit) was raised.

A:
Thanks for your inquiry.
Pls check our answer:
The kit csb-e13024r kit uses two antibodies, mouse monoclonal antibody pre-coated in the plate and goat polyclonal antibody with biotin label. Both of the antibodies are use mIU/mL as the unit.
The kit is quantitatively detected the content of Alanine aminotransferase(ALT).
As for the published reference, pls check this link: https://www.cusabio.com/manage/index.php?action=class&todo=content&m=248&page=1&year=1&code=CSB-E13024r
Any question, pls feel free to contact me.Pls let me know if you have any further questions. Thank you.
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靶点详情

  • 功能：
    Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
  • 基因功能参考文献：
    1. Results indicate that AZD4619 increased alanine aminotransferase (ALT1) protein expression in a dose-dependent manner in human, but not in rat hepatocytes. PMID: 27430334
    2. Data suggest that both Alt1 and Alt2 are induced in liver during gluconeogenesis and in diabetes (Goto-Kakizaki rats); in hepatocytes, both Alt1 and Alt2 are induced by glucocorticoid dexamethasone and are suppressed by insulin. PMID: 25865565
    3. Although ALT and GMT activities and TNF-alpha and IL-6 concentrations were higher in male rats histopathological findings have shown slightly more advanced fibrotic and inflammatory changes in female rats. PMID: 17582939
    4. Report the early effects of short-term dexamethasone administration on hepatic and serum alanine aminotransferase in the rat. PMID: 18850354
    5. Protein levels are significantly elevated and correlate with activity, providing the molecular basis for the elevated total serum enzyme activity in liver toxicity. PMID: 19085960

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
  • 组织特异性：
    Liver, heart, skeletal muscle, etc.
  • 数据库链接：

    KEGG: rno:81670

    STRING: 10116.ENSRNOP00000044411

    UniGene: Rn.6318

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