Rat 6-keto-PGF1α ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO高灵敏度大鼠6-酮前列腺素F1α（6-keto-PGF1α）定量检测试剂盒，采用双抗体夹心法原理，适用于血清、血浆、组织匀浆及细胞培养上清液等多种样本类型，检测范围为31.25-2000 pg/mL，可精准测定微量目标物含量。6-keto-PGF1α是前列环素（PGI2）的稳定水解代谢产物，其水平能间接反映血管内皮细胞功能状态，常用于研究血管舒缩调节、炎症反应及血栓形成等生理病理过程。试剂盒操作便捷，可在标准实验室条件下完成检测，提供高特异性抗体对及优化的反应体系，支持心血管疾病模型、药物干预实验及炎症机制等基础研究，尤其适用于探索氧化应激、内皮损伤或抗血小板药物药效评价等领域。通过检测不同样本中6-keto-PGF1α的动态变化，可为血管功能相关科研课题提供可靠数据支持。
别名：

6-keto-PGF1α,6-keto-PGF1 alpha
缩写：

6-keto-PGF1α
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell culture supernates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat 6-keto-PGF1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	88
	Range %	82-95
1:4	Average %	90
	Range %	85-95
1:8	Average %	92
	Range %	84-97

回收率：

The recovery of rat 6-keto-PGF1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-100
EDTA plasma (n=4)	98	93-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.