Rat 5-hydroxyindolacetic acid,5-HIAA ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠5羟基吲哚乙酸(5-HIAA)酶联免疫试剂盒（CSB-E13983r）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的5-HIAA含量。5-HIAA即5-羟吲哚乙酸，是血清素的主要代谢产物。它在神经生物学研究中是重要指标，可反映血清素的代谢水平。其研究机制主要围绕血清素能神经功能展开，通过检测体内含量变化，辅助探究神经系统疾病如抑郁、焦虑等发病机制。试剂盒检测范围为25 ng/mL-400 ng/mL，常用于神经递质代谢研究、神经退行性疾病模型评估以及精神类药物作用机制探索等领域。适用于实验室开展神经生物学、药理学或毒理学研究，例如评估药物干预对5 - 羟色胺代谢通路的影响，或探究特定病理状态下神经递质动态变化等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

5-HIAA
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

25 ng/mL-400 ng/mL
灵敏度：

25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat 5-HIAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-100
1:2	Average %	93
	Range %	88-97
1:4	Average %	98
	Range %	94-100
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of rat 5-HIAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	94	91-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.