Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA kit

Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA kit

产品详情

引用文献

产品评价

靶点详情

产品详情

引用文献

产品评价

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

大鼠5` -核苷酸酶，胞膜(NT5E/CD73)酶联免疫试剂盒
货号：

CSB-E14100r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
引用文献
靶点详情

产品描述：

CUSABIO专为大鼠样本设计的NT5E/CD73酶联免疫检测试剂盒（货号：CSB-E14100r），通过双抗体夹心法定量检测血清、血浆、组织匀浆及细胞培养上清液中的5'-核苷酸酶（CD73）水平。CD73作为胞膜外酶，通过催化AMP生成腺苷参与嘌呤代谢调控，其表达水平与炎症反应、肿瘤免疫逃逸及心血管疾病等病理过程密切相关。试剂盒采用高特异性配对抗体，检测灵敏度达0.625 mIU/mL，线性范围为0.625-40 mIU/mL，可精确捕捉生物样本中微量CD73的动态变化。适用于评估CD73在肿瘤微环境、神经退行性疾病模型或代谢性疾病研究中的表达特征，也可用于药物干预实验中的生物标志物检测。实验全程仅需标准ELISA操作流程，96孔板预包被设计便于批量样本分析，配套标准品及显色系统确保实验稳定性和重复性，为研究腺苷信号通路及其相关疾病机制提供可靠的科研工具。
别名：

Nt5e ELISA kit; Nt5 ELISA kit; Nte5'-nucleotidase ELISA kit; 5'-NT ELISA kit; EC 3.1.3.5 ELISA kit; Ecto-5'-nucleotidase ELISA kit; CD antigen CD73 ELISA kit
缩写：

NT5E/CD73
Uniprot No.：

P21588
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell culture supernates
检测范围：

0.625 mIU/mL-40 mIU/mL
灵敏度：

0.156 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CD73 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-96
1:2	Average %	87
	Range %	82-95
1:4	Average %	90
	Range %	85-97
1:8	Average %	93
	Range %	86-101

回收率：

The recovery of rat CD73 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-101
EDTA plasma (n=4)	91	86-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
40	2.504	2.416	2.460	2.358
20	1.933	1.824	1.879	1.777
10	1.386	1.283	1.335	1.233
5	0.814	0.749	0.782	0.680
2.5	0.424	0.397	0.411	0.309
1.25	0.228	0.213	0.221	0.119
0.625	0.154	0.145	0.150	0.048
0	0.103	0.101	0.102

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Biochemical studies on the effect of prostaglandin inhibitors and willow bark extract on liver cirrhosis induced by acetylsalicylate in rats Doha Mohammad Beltag,Journal of Medical and Life Science,2022

功能：

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
亚细胞定位：

Cell membrane; Lipid-anchor, GPI-anchor.
蛋白家族：

5'-nucleotidase family
数据库链接：

STRING: 10116.ENSRNOP00000015057

UniGene: Rn.40132

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.