Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit

产品详情

产品描述：

大鼠α1酸性糖蛋白(α1-AGP)酶联免疫试剂盒（CSB-E12730r）为竞争法ELISA试剂盒，定量检测血清、血浆、尿液、组织培养上清液、组织匀浆样本中的ORM1含量。ORM1即阿片受体μ样1，是一种重要的药物靶点。研究发现它参与痛觉调节、情绪调控等生理过程。其机制主要在于与内源性配体结合后，通过一系列信号转导影响细胞功能，在镇痛、精神疾病治疗等方面有潜在研究价值。试剂盒检测范围为3.125 ng/mL-800 ng/mL，具备高灵敏度和特异性，适用于炎症相关机制研究、药物干预效果评价或疾病动物模型中的生物标志物分析等科研场景，实验操作流程标准化，配套试剂完整，可满足实验室常规检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Orm1Alpha-1-acid glycoprotein ELISA kit; Orosomucoid ELISA kit; OMD ELISA kit
缩写：

ORM1
Uniprot No.：

P02764
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, urine, cell culture supernates, tissue homogenates
检测范围：

3.125 ng/mL-800 ng/mL
灵敏度：

3.125 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat α1-AGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	95
	Range %	85-105
1:2000	Average %	89
	Range %	81-98
1:4000	Average %	90
	Range %	82-97
1:8000	Average %	95
	Range %	83-98

回收率：

The recovery of rat α1-AGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	95	90-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
基因功能参考文献：
1. fatigue upregulates the level of ORM1, which in turn functions as an anti-fatigue protein to enhance muscle endurance via the CCR5 pathway. PMID: 26740279
2. context-dependent nature of ORM1 angioregulatory function is further demonstrated in the CAM assay where ORM1 stimulates developmental angiogenesis without exerting any inhibitory activity PMID: 22916107
3. alpha-1 acid glycoprotein expression is increased by retinoids at the transcriptional level through two distinct DR1 retinoic acid responsive elements PMID: 15157739
4. Surprisingly, AGP-over-expressing mice were not protected against renal ischemia-reperfusion injury. PMID: 15502707
5. Orm1 gene expression highly induced in cardiomyocytes overexpressing mineralocorticoid receptor and treated with aldosterone. PMID: 17234708
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亚细胞定位：

Secreted.
蛋白家族：

Calycin superfamily, Lipocalin family
数据库链接：

KEGG: rno:24614

STRING: 10116.ENSRNOP00000010454

UniGene: Rn.10295