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Rabbit β-Thromboglobulin,β-TG ELISA Kit

  • 中文名称:
    兔子β血小板球蛋白/β血栓环蛋白(β-TG)酶联免疫试剂盒
  • 货号:
    CSB-E09285Rb
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:
    兔子β血小板球蛋白/β血栓环蛋白(β-TG)酶联免疫试剂盒(CSB-E09285Rb)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的β-TG含量。试剂盒检测范围为12.5 ng/mL-800 ng/mL,适用于血小板功能评估、血栓形成机制研究、心血管疾病模型病理过程探索等科研领域。为体外研究β - TG在止血、炎症反应及血管内皮损伤中的生物学作用提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    beta-Thromboglobulin, beta-TG
  • 缩写:
    β-TG
  • 种属:
    Oryctolagus cuniculus (Rabbit)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    12.5 ng/mL-800 ng/mL
  • 灵敏度:
    3.12 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays):CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit β-TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 91
    Range % 86-95
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 91
    Range % 85-97
    1:8 Average % 97
    Range % 91-103
  • 回收率:
    The recovery of rabbit β-TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 89-98
    EDTA plasma (n=4) 97 90-100
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    800 2.801 2.869 2.835 2.748
    400 2.484 2.398 2.441 2.354
    200 1.716 1.768 1.742 1.655
    100 0.944 0.968 0.956 0.869
    50 0.454 0.468 0.461 0.374
    25 0.256 0.248 0.252 0.165
    12.5 0.147 0.151 0.149 0.062
    0 0.085 0.089 0.087  
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

平均分:
5.0分 - 3 个评价

样品类型:血浆(抗凝剂) 柠檬酸三钠

样品信息:其他 兔

稀释比:没有稀释

产品评价: 我用CSB-E09285Rb检测正常兔血清中β-TG含量,标准曲线的R2可以做到高达0.9921,标准曲线符合要求,样品OD值其中之一0.264、0.268,可以做到平行,检测结果准确且完美。

By 王老师

样品类型:血浆(抗凝剂) 柠檬酸钠

样品信息:其他 兔

稀释比:没有稀释

产品评价: 我用CSB-E09285Rb检测正常兔血清中β-TG含量,标准曲线的R2可以做到大于0.99,样品OD值符合范围,检测结果准确且完美。

By 花老师

样品类型:血清

样品信息:其他 兔

稀释比:没有稀释

产品评价: 我用CSB-E09285Rb检测正常兔血清中β-TG含量,标准曲线的R2可以做到高达0.9997,标准曲线符合要求,样品OD值符合要求,可以做到平行,检测结果准确且完美。

By 王老师

相关问答

 常见问题解答
Q:

I would like to redirect another rabbit assay to your assay because it is based on antibodies against the human sequence and not rabbit.
I understand that immunogen information for your kits is not normally revealed however if we are going to redirect another assay to your kit we need to know that the antibodies in your kit are directed against the rabbit sequence. If not and they are against a different species then please reveal that species and how cross reactivity has been confirmed.

A:
Thanks for your inquiry.
About Rabbit beta-Thromboglobulin, pls refer to:
https://www.ncbi.nlm.nih.gov/protein/XP_008266105.1
For this kit CSB-E09285Rb, the standard is 293 cell-derived recombinant protein.
Pls let me know if you have any further questions. Thank you.