Rabbit tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专用于科研领域的兔子基质金属蛋白酶抑制因子1（TIMP-1）酶联免疫检测试剂盒（货号：CSB-E06935Rb），基于竞争法原理实现定量分析，可检测浓度范围为15.6 pg/mL至1000 pg/mL。TIMP-1是调控细胞外基质代谢的关键因子，通过抑制金属蛋白酶活性参与组织修复、纤维化及肿瘤微环境调节等生物学过程。该试剂盒适用于血清、血浆、细胞培养上清液及组织匀浆样本的检测，其高灵敏度设计可精准识别低丰度靶标，满足体外实验中对TIMP-1表达水平的动态监测需求，例如在细胞模型或动物实验中探究炎症反应、组织重塑或疾病进展相关机制。检测流程通过标准品梯度稀释建立定量曲线，结合特异性抗体实现目标蛋白的定量分析，具有操作便捷、重复性强的特点，尤其适用于复杂生物样本中TIMP-1的科研级定量研究。
别名：

TIMP1 ELISA Kit; Metalloproteinase inhibitor 1 ELISA Kit; Tissue inhibitor of metalloproteinases 1 ELISA Kit; TIMP-1 ELISA Kit
缩写：

TIMP1
Uniprot No.：

P20614
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, cell culture supernates , tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TIMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	90
	Range %	85-97
1:200	Average %	102
	Range %	98-108
1:400	Average %	98
	Range %	94-102
1:800	Average %	88
	Range %	84-93

回收率：

The recovery of rabbit TIMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	92	88-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.
基因功能参考文献：
1. Hemoperfusion could obviously reduce oxidative stress and the expression levels of MMP-2, MMP-9 and TIMP-1 in rabbits with acute paraquat poisoning. PMID: 22801080
亚细胞定位：

Secreted.
蛋白家族：

Protease inhibitor I35 (TIMP) family
数据库链接：

KEGG: ocu:100009047

STRING: 9986.ENSOCUP00000009228

UniGene: Ocu.178