Rabbit matrix metalloproteinase 9,MMP-9 ELISA Kit

Rabbit matrix metalloproteinase 9,MMP-9 ELISA Kit

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中文名称：

兔子基质金属蛋白酶9(MMP-9)酶联免疫试剂盒
货号：

CSB-E06933Rb
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

本产品是专为科研领域设计的兔源基质金属蛋白酶9（MMP-9）定量检测试剂盒（货号：CSB-E06933Rb），采用竞争法原理精准测定血清、血浆、细胞培养上清及组织匀浆中的靶蛋白浓度。MMP-9作为基质金属蛋白酶家族成员，在细胞外基质重塑、炎症反应调控及肿瘤侵袭转移中发挥关键作用，其动态变化常作为组织修复、慢性炎症及肿瘤微环境研究的分子标志物。试剂盒具备23.5-1500 pg/mL的宽检测范围，可灵敏捕捉样本中微量MMP-9的表达差异，适用于体外实验中炎症机制研究、肿瘤细胞侵袭模型分析、药物干预效果评估等基础科研场景。操作流程兼容常规酶标仪平台，通过预包被特异性抗体的96孔板实现标准化检测，配套校准品及优化反应体系确保数据可靠性，为心血管疾病、纤维化病理、创伤愈合等研究领域提供高效检测工具。
别名：

MMP9Matrix metalloproteinase-9 ELISA Kit; MMP-9 ELISA Kit; EC 3.4.24.35 ELISA Kit; 92 kDa gelatinase ELISA Kit; 92 kDa type IV collagenase ELISA Kit; Gelatinase B ELISA Kit; GELB ELISA Kit
缩写：

MMP9
Uniprot No.：

P41246
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

23.5 pg/mL-1500 pg/mL
灵敏度：

5.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	99
	Range %	96-102
1:40	Average %	85
	Range %	92-99
1:80	Average %	101
	Range %	98-104
1:160	Average %	85
	Range %	82-89

回收率：

The recovery of rabbit MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	93-99
EDTA plasma (n=4)	100	96-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1500	0.129	0.127	0.128
750	0.226	0.238	0.232
375	0.379	0.370	0.375
187.5	0.572	0.565	0.569
94	0.799	0.785	0.792
47	0.937	0.987	0.962
23.5	1.148	1.168	1.158
0	1.606	1.634	1.620

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Chemical (Alkali) Burn-Induced Neurotrophic Keratitis Model in New Zealand Rabbit Investigated Using Medical Clinical Readouts and In Vivo Confocal Microscopy (IVCM) M Vohra,Cells,2024
Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits MiljanaBacevic,Archives of Oral Biology,2019

功能：

Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
基因功能参考文献：
1. The present study demonstrated the ability of 30 and 100 ng/ml TIMP3 to attenuate migration and proliferation, and to inhibit the activity of MMP2, MMP9 and TNFalpha secretion of NA SMCs. In conclusion, TIMP3 may be considered a potential therapeutic drug for use in a novel drugeluting stent, to attenuate the progressive dilation of the aortic NA. PMID: 29956789
2. Expression of MMP-9 increased after cerebral aneurysm induction, peaking at week 3, leading to reduced smooth muscle cell number, damaged endothelial cells, and damage to the aneurysm wall elastic layer. PMID: 24534031
3. Inflammatory factors such as TNF-alpha can stimulate MMP-2/9 activity in corneal epithelium cells. This may be a potential manipulating mechanism of MMP expression in the pathogenesis of corneal diseases PMID: 26125840
4. Therefore, it was reasonable to speculate that the increased expression of VEGF and MMP-9 in residual hepatic tumor cells and tumor angiogenesis post-embolization would be responsible for the increased metastatic potentiality and invasiveness. PMID: 23392714
5. Performing minimally invasive surgical procedures in the early stages of intracerebral hemorrhage significantly decreases MMP-9. PMID: 23561043
6. Increased expression of MMP-9 in spinal cord follows cervical spondylotic myelopathy. PMID: 22673181
7. Hemoperfusion could obviously reduce oxidative stress and the expression levels of MMP-2, MMP-9 and TIMP-1 in rabbits with acute paraquat poisoning. PMID: 22801080
8. Exposure to long periods of light irrespective of its characteristics leads to the increased expression of some matrix metalloprpteases. PMID: 20238014
9. In experimental syringomyelia, MMP-9 plays an important role in causing edema in the presyrinx state. PMID: 21092471
10. Tongxinluo can inhibit the expression of MMP-3 and 9 and increase the expression of PPARgamma in atherosclerotic rabbits. PMID: 19778818
11. MMP promoter activation occurs as a function of thoracic aorta wall tension in transgenic mice PMID: 19375723
12. TGF-beta mediated MMP-9 induction may be regulated by the NF-kappaB, Smad3, and JNK pathways, whereas the IL-1beta mediated induction may be regulated by the NF-kappaB and p38 pathways. PMID: 19626678
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亚细胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Peptidase M10A family
组织特异性：

Osteoclasts.
数据库链接：

KEGG: ocu:100008993

STRING: 9986.ENSOCUP00000025066

UniGene: Ocu.1773

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.