Rabbit matrix metalloproteinase 3,MMP-3 ELISA kit

Rabbit matrix metalloproteinase 3,MMP-3 ELISA kit

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中文名称：

兔子基质金属蛋白酶3(MMP-3)酶联免疫试剂盒
货号：

CSB-E09269Rb
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO兔源基质金属蛋白酶3（MMP-3）酶联免疫检测试剂盒（货号：CSB-E09269Rb），采用双抗体夹心法原理实现定量分析，适用于检测血清、血浆及组织匀浆样本中MMP-3的蛋白表达水平。MMP-3作为基质金属蛋白酶家族成员，在细胞外基质降解、组织重塑及炎症反应中起关键作用，其异常表达与关节炎、纤维化疾病及肿瘤侵袭等病理过程密切相关。该试剂盒检测灵敏度达0.156 ng/mL，线性范围为0.156-10 ng/mL，通过预包被特异性捕获抗体与标记检测抗体的协同作用，可精准识别天然和重组兔MMP-3蛋白。实验操作全程约需4.5小时，包含标准品、检测抗体及显色系统等核心组分，适用于体外培养细胞、动物模型组织等科研场景中MMP-3的动态监测，为研究结缔组织代谢调控、炎症性疾病机制及药物干预效果提供可靠工具，实验结果需使用酶标仪在450 nm波长下读取。
别名：

MMP3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; Transin-1 ELISA Kit
缩写：

MMP3
Uniprot No.：

P28863
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	84
	Range %	80-94
1:10	Average %	97
	Range %	91-105
1:20	Average %	93
	Range %	85-97
1:40	Average %	94
	Range %	86-99

回收率：

The recovery of rabbit MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-98
EDTA plasma (n=4)	89	85-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.794	2.694	2.744	2.593
5	2.244	2.144	2.194	2.043
2.5	1.424	1.324	1.374	1.223
1.25	0.854	0.824	0.839	0.688
0.625	0.546	0.536	0.541	0.390
0.312	0.361	0.351	0.356	0.205
0.156	0.230	0.220	0.225	0.074
0	0.152	0.149	0.151

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Ginsenoside-Rb1 prevents bone cartilage destruction through down-regulation of p-Akt, p-P38, and p-P65 signaling in rabbit MA Hossain,Phytomedicine,2023

功能：

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
基因功能参考文献：
1. Compromised autophagy may be related to the osteoarthritis progression; JNK and p38 MAPKs up-regulate MMP3 and down-regulate autophagy. PMID: 26873249
2. the total content and active forms of matrix metalloproteinases (MMPs)-1, -3, and -13 was measured to assess the potential role of the mechanical environment in regulation of matrix turnover by ligament fibroblasts. PMID: 23879595
3. chitosan-pDNA nanoparticles encoding shRNA targeting MMP-3 and -13 had great potential in silencing the dedifferentiation-related genes for regenerating prolonged and endurable cartilage. PMID: 23520014
4. Ulinastatin effectively inhibited the increased expression of MMP-2, MMP-3, and iNOS in degenerated NP cells induced by IL-1beta in vitro. PMID: 22853554
5. Exposure to long periods of light irrespective of its characteristics leads to the increased expression of some matrix metalloprpteases. PMID: 20238014
6. Tongxinluo can inhibit the expression of MMP-3 and 9 and increase the expression of PPARgamma in atherosclerotic rabbits. PMID: 19778818
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亚细胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Peptidase M10A family
数据库链接：

KEGG: ocu:100009111

STRING: 9986.ENSOCUP00000013084

UniGene: Ocu.1964

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.