Rabbit matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA Kit

Rabbit matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA Kit

产品详情

产品评价

靶点详情

产品详情

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

兔基质金属蛋白酶2/明胶酶A(MMP-2/Gelatinase A)酶联免疫试剂盒
货号：

CSB-E10373Rb
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
相关产品
靶点详情

产品描述：

CUSABIO兔基质金属蛋白酶2/明胶酶A（MMP-2/Gelatinase A）特异性定量检测试剂盒，采用双抗体夹心ELISA技术，可精准分析血清、血浆及组织匀浆样本中MMP-2的蛋白表达水平。MMP-2作为锌依赖性内肽酶家族成员，通过降解IV型胶原等细胞外基质成分参与组织重塑、血管生成和病理过程，其活性与肿瘤侵袭转移、慢性炎症及纤维化疾病密切相关。试剂盒检测灵敏度为1.56 ng/mL，线性范围覆盖1.56-100 ng/mL，提供预包被抗体的96孔板、标准品、检测抗体及显色系统，适用于体外定量检测兔源样本中天然与重组MMP-2蛋白。该试剂盒在3小时内即可完成检测流程，支持科研领域对动物疾病模型（如关节炎、肿瘤移植模型）的分子机制研究，或用于细胞培养上清、组织提取液中MMP-2动态变化的分析，为探索基质代谢调控、药物干预效果评估提供可靠工具。
别名：

MMP2 ELISA Kit; 72 kDa type IV collagenase ELISA Kit; EC 3.4.24.24 ELISA Kit; 72 kDa gelatinase ELISA Kit; Gelatinase A ELISA Kit; Matrix metalloproteinase-2 ELISA Kit; MMP-2) [Cleaved into: PEX] ELISA Kit
缩写：

MMP2
Uniprot No.：

P50757
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	89
	Range %	85-95
1:20	Average %	96
	Range %	90-105
1:40	Average %	93
	Range %	85-99
1:80	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-103
EDTA plasma (n=4)	92	87-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.506	2.531	2.519	2.419
50	2.048	2.005	2.027	1.927
25	1.674	1.691	1.683	1.583
12.5	1.184	1.207	1.196	1.096
6.25	0.882	0.879	0.881	0.781
3.12	0.554	0.543	0.549	0.449
1.56	0.306	0.314	0.310	0.210
0	0.102	0.097	0.100

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues.; PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrin alpha-v/beta-3 on the surface of blood vessels.
基因功能参考文献：
1. These results show that MMP-2 activates the EGFR and triggers downstream signaling pathways increasing Reactive Oxygen Species formation and promoting vasoconstriction. PMID: 30029165
2. The present study demonstrated the ability of 30 and 100 ng/ml TIMP3 to attenuate migration and proliferation, and to inhibit the activity of MMP2, MMP9 and TNFalpha secretion of NA SMCs. In conclusion, TIMP3 may be considered a potential therapeutic drug for use in a novel drugeluting stent, to attenuate the progressive dilation of the aortic NA. PMID: 29956789
3. Selenium suppressed high-fat diet-induced MMP2 over-expression in vivo by improving lipid metabolism. PMID: 28595793
4. Repetitive mechanical stimulation of tendon cells results in a small increase in matrix metalloproteinase2 levels, but it appears unlikely that serum matrix metalloproteinase2 will be a useful indicator of tendon overuse injury. PMID: 26909661
5. Inflammatory factors such as TNF-alpha can stimulate MMP-2/9 activity in corneal epithelium cells. This may be a potential manipulating mechanism of MMP expression in the pathogenesis of corneal diseases PMID: 26125840
6. Results provide evidence that MMP-2 bears the potentiality to cleave alpha-DG enriched from rabbit skeletal muscle indicating that this degradation indeed might also occur in vivo. PMID: 25483986
7. These data suggest that OxyHb suppresses K V currents through both reactive oxygen species-dependent and independent pathways involving MMP activation. PMID: 25366605
8. In conclusion, MMP-2 could be responsible for the proteolysis of dystrophin. PMID: 24791785
9. Castor oil polymer induces bone formation with high matrix metalloproteinase-2 expression. PMID: 23670892
10. MMP2 spinal cord expression is increased in cervical spondylotic myelopathy. PMID: 22673181
11. Ulinastatin effectively inhibited the increased expression of MMP-2, MMP-3, and iNOS in degenerated NP cells induced by IL-1beta in vitro. PMID: 22853554
12. Hemoperfusion could obviously reduce oxidative stress and the expression levels of MMP-2, MMP-9 and TIMP-1 in rabbits with acute paraquat poisoning. PMID: 22801080
13. Exposure to long periods of light irrespective of its characteristics leads to the increased expression of some matrix metalloprpteases. PMID: 20238014
14. The RNA interference targeting COX-2 can effectively inhibit the expression of COX-2 and MMP-2 in IL-1alpha stimulated rabbit corneal stromal cells in vitro. PMID: 19175164
15. Our results strongly suggest that ischaemic postconditioning may exert part of its cardioprotective effects through the inhibition of MMP-2 activity. PMID: 19880538
显示更多

收起更多
亚细胞定位：

Secreted, extracellular space, extracellular matrix. Membrane. Nucleus.
蛋白家族：

Peptidase M10A family
数据库链接：

KEGG: ocu:100009000

STRING: 9986.ENSOCUP00000005803

UniGene: Ocu.6274

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.