Rabbit matrix metalloproteinase 1,MMP-1 ELISA kit

Rabbit matrix metalloproteinase 1,MMP-1 ELISA kit

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中文名称：

兔基质金属蛋白酶1(MMP-1)酶联免疫试剂盒
货号：

CSB-E09744Rb
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO兔基质金属蛋白酶1（MMP-1）酶联免疫检测试剂盒（货号CSB-E09744Rb），适用于定量检测血清、血浆及组织匀浆样本中的MMP-1含量。MMP-1作为基质金属蛋白酶家族成员，在细胞外基质降解、组织重塑及修复过程中发挥重要作用，其表达水平与炎症反应、纤维化等病理过程密切相关。试剂盒采用双抗体夹心法原理实现精准定量检测，灵敏度达0.156 ng/mL，检测线性范围为0.156-10 ng/mL，可覆盖绝大多数生物样本中MMP-1的浓度区间。实验操作全程可在常规实验室开展，兼容酶标仪检测系统，支持批量样本的高通量分析。该试剂盒适用于基础医学研究领域，如探究心血管疾病、关节炎或皮肤纤维化等疾病模型中MMP-1的调控机制，也可用于药物开发过程中对MMP-1抑制剂的体外效果评估。需注意本产品仅供科学研究使用，配套提供标准品、预包被板及检测所需试剂组分，实验流程标准化且重复性良好，建议实验前通过预实验优化样本稀释比例以确保检测结果准确性。
别名：

MMP1Interstitial collagenase ELISA Kit; EC 3.4.24.7 ELISA Kit; Matrix metalloproteinase-1 ELISA Kit; MMP-1 ELISA Kit
缩写：

MMP1
Uniprot No.：

P13943
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	104
	Range %	97-109
1:4	Average %	88
	Range %	82-92
1:8	Average %	98
	Range %	91-102

回收率：

The recovery of rabbit MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	82-92
EDTA plasma (n=4)	102	92-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.590	2.545	2.568	2.466
5	1.963	1.922	1.943	1.841
2.5	1.435	1.400	1.418	1.316
1.25	0.841	0.818	0.830	0.728
0.625	0.448	0.427	0.438	0.336
0.312	0.328	0.317	0.323	0.221
0.156	0.248	0.242	0.245	0.143
0	0.101	0.103	0.102

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X.
基因功能参考文献：
1. These results suggest that PEP-1-SIRT2 promotes matrix metalloproteinases-induced dedifferentiation via ERK signaling in articular chondrocytes. . PMID: 27697532
2. the total content and active forms of matrix metalloproteinases (MMPs)-1, -3, and -13 was measured to assess the potential role of the mechanical environment in regulation of matrix turnover by ligament fibroblasts. PMID: 23879595
3. Circulating smoke components, including acrolein, contribute to vascular diseases through enhanced MMP-1 and decreased TIMP-3 secretion. PMID: 21742783
亚细胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Peptidase M10A family
数据库链接：

KEGG: ocu:100009110

STRING: 9986.ENSOCUP00000015428

UniGene: Ocu.2695

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.