Rabbit intercellular adhesion molecule 1,ICAM-1/CD54 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

兔子细胞间粘附分子1(ICAM-1/CD54)酶联免疫试剂盒（CSB-E06974Rb）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的ICAM-1/CD54含量。ICAM-1/CD54是重要细胞黏附分子。其背景为在多种细胞表面表达，参与免疫应答、炎症反应等生理病理过程。研究机制上，它能介导细胞间黏附与信号传导，异常表达与多种疾病如炎症性疾病、肿瘤转移等密切相关，是潜在治疗靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，兔类动物模型中炎症相关疾病机制研究、免疫调节药物开发评估、感染病理进程分析、心血管疾病模型、自身免疫性疾病研究、病毒/细菌感染实验中的生物标志物动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

ICAM-1/CD54
Uniprot No.：

U3KPG6
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ICAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	94
	Range %	91-98
1:10	Average %	99
	Range %	91-106
1:20	Average %	87
	Range %	82-94
1:40	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit ICAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	100	96-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.