Rabbit follicle-stimulating hormone,FSH ELISA Kit

Rabbit follicle-stimulating hormone,FSH ELISA Kit

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中文名称：

兔子促卵泡素(FSH)酶联免疫试剂盒
货号：

CSB-E06918Rb
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CSB-E06918Rb兔子促卵泡素(FSH)酶联免疫试剂盒采用双抗体夹心法原理开发，专为定量检测兔血清、血浆或组织匀浆样本中的FSH水平而设计。FSH作为垂体前叶分泌的糖蛋白激素，在雌性动物中可促进卵泡成熟及雌激素合成，在雄性动物中则参与调控精子生成过程，是生殖生理研究的重要靶标。本试剂盒通过预包被特异性抗体捕获样本中的FSH分子，结合酶标抗体形成复合物后进行显色反应，其颜色深浅与FSH浓度呈正相关，检测灵敏度达到1.56 mIU/mL，线性范围覆盖1.56-100 mIU/mL，可精准检测生理及实验干预条件下的激素水平波动。该产品适用于科研领域中对兔子生殖内分泌系统的机制研究、繁殖调控实验评估或药物对性腺功能影响的体外分析，操作全程仅需常规离心处理的液态样本，实验流程标准化且重复性良好，96孔板规格可同时处理多样本检测需求，配套提供标准品、显色液及终止液等完整组分，为研究者提供稳定可靠的实验解决方案。
别名：

FSHBFollitropin subunit beta ELISA Kit; Follicle-stimulating hormone beta subunit ELISA Kit; FSH-B ELISA Kit; FSH-beta ELISA Kit; Follitropin beta chain ELISA Kit
缩写：

FSH
Uniprot No.：

Q6IY73
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 mIU/mL-100 mIU/mL
灵敏度：

0.39 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	99
	Range %	91-105
1:4	Average %	103
	Range %	92-110
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	96	90-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
100	2.885	2.878	2.882	2.789
50	2.346	2.221	2.284	2.191
25	1.619	1.603	1.611	1.518
12.5	0.951	0.933	0.942	0.849
6.25	0.468	0.451	0.460	0.367
3.12	0.255	0.243	0.249	0.156
1.56	0.159	0.149	0.154	0.061
0	0.096	0.090	0.093

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Effects of Treatment with Nauclea latifolia Root Decoction on Sexual Behavior and Reproductive Functions in Male Rabbits Alabi QK.et al,J Diet Suppl,2017

功能：

Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
亚细胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
数据库链接：

KEGG: ocu:100008946

STRING: 9986.ENSOCUP00000016404

UniGene: Ocu.3174

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.