Rabbit cholesteryl ester transfer protein,CETP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO兔子胆固醇酯转移蛋白（CETP）酶联免疫检测试剂盒（货号：CSB-E06841Rb），采用竞争法原理实现定量分析，适用于检测血清、血浆及组织匀浆样本中的CETP水平，检测范围为1.56-100 ng/mL。CETP是胆固醇逆向转运途径中的关键蛋白，通过介导胆固醇酯与甘油三酯在脂蛋白间的交换，调节高密度脂蛋白（HDL）和低密度脂蛋白（LDL）的代谢平衡，其表达水平与动脉粥样硬化、心血管疾病等脂代谢异常密切相关。本试剂盒通过特异性抗体与样本中CETP的竞争性结合反应实现精准检测，可为脂质代谢机制研究、动物模型评估及药物开发中CETP活性调控分析提供可靠工具。科研人员可将其应用于高脂血症模型兔的血清标志物分析、脂蛋白代谢通路研究或针对CETP抑制剂的药效学评价，实验操作便捷，检测重复性良好，满足体外样本中低浓度CETP的定量需求。
别名：

CETPCholesteryl ester transfer protein ELISA kit; Lipid transfer protein I ELISA kit; Fragment ELISA kit
缩写：

CETP
Uniprot No.：

P22687
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CETP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	99
	Range %	94-102
1:200	Average %	97
	Range %	94-100
1:400	Average %	83
	Range %	80-86
1:800	Average %	91
	Range %	87-95

回收率：

The recovery of rabbit CETP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	97-103
EDTA plasma (n=4)	84	81-89

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Involved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, among lipoprotein particles. Allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transport of triglyceride from VLDL to HDL. Regulates the reverse cholesterol transport, by which excess cholesterol is removed from peripheral tissues and returned to the liver for elimination.
亚细胞定位：

Secreted.
蛋白家族：

BPI/LBP/Plunc superfamily, BPI/LBP family
组织特异性：

Expressed by the liver.
数据库链接：

STRING: 9986.ENSOCUP00000011306

UniGene: Ocu.1994