Rabbit cardiac troponin Ⅰ,cTn-ⅠELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

兔心肌肌钙蛋白Ⅰ(cTn-Ⅰ)酶联免疫试剂盒（CSB-E11286Rb）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNNI3含量。TNNI3是重要的心肌肌钙蛋白亚基，在心肌收缩和舒张调节中发挥关键作用。其异常与多种心脏疾病密切相关，如心肌梗死、心肌病等。科研人员通过研究其表达、结构及功能机制，探索基于TNNI3的诊断标志物和治疗靶点。试剂盒检测范围为47 pg/mL-3000 pg/mL，该产品适用于基础科研中与心肌损伤相关的动物模型研究，如心肌缺血再灌注损伤、药物心脏毒性评估或心血管疾病机制探讨等领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

TNNI3 ELISA Kit; Troponin I ELISA Kit; cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit
缩写：

TNNI3
Uniprot No.：

P02646
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

47 pg/mL-3000 pg/mL
灵敏度：

11.75 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-92
1:2	Average %	96
	Range %	92-99
1:4	Average %	94
	Range %	89-98
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of rabbit cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-94
EDTA plasma (n=4)	89	85-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
蛋白家族：

Troponin I family