Rabbit basic fibroblast growth factor,bFGF ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

兔子碱性成纤维细胞生长因子(bFGF)酶联免疫试剂盒（CSB-E06938Rb）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGF2含量。FGF2即成纤维细胞生长因子2，在细胞的增殖、分化及血管生成等生理过程中发挥重要作用。其研究机制方面，FGF2通过与细胞膜上的受体结合，激活下游信号通路，影响细胞行为，在肿瘤、心血管疾病等研究领域受关注。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于体外培养细胞模型、动物组织样本或体液样本的bFGF含量检测，为研究组织再生机制、肿瘤微环境调控或炎症反应等科研领域提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

FGF2 ELISA Kit; Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2 ELISA Kit; Fragment ELISA Kit
缩写：

FGF2
Uniprot No.：

P48799
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

7.8 pg/mL-500 pg/mL
灵敏度：

1.95 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-90
1:2	Average %	104
	Range %	100-108
1:4	Average %	85
	Range %	81-89
1:8	Average %	99
	Range %	95-102

回收率：

The recovery of rabbit bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	83	80-86
EDTA plasma (n=4)	97	94-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
亚细胞定位：

Secreted. Nucleus.
蛋白家族：

Heparin-binding growth factors family
数据库链接：

STRING: 9986.ENSOCUP00000000416

UniGene: Ocu.1874