Rabbit apolipoprotein B100 (Apo-B100) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

兔载脂蛋白B100(Apo-B100)酶联免疫试剂盒（CSB-E09805Rb）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Apo-B100含量。Apo-B100是低密度脂蛋白（LDL）的主要结构蛋白，参与脂质和胆固醇代谢，与动脉粥样硬化密切相关。研究显示，Apo-B100通过冷冻电镜、AlphaFold2和分子动力学方法解析其结构，揭示其与LDL受体（LDLR）的相互作用机制，为开发针对胆固醇代谢异常的新疗法提供新视角。试剂盒检测范围为78 ng/mL-5000 ng/mL，灵敏度为19.5 ng/mL。适用于基础科研中脂代谢机制研究、动脉粥样硬化病理模型分析、药物干预对脂蛋白合成影响的评估等方向，为心血管疾病相关动物实验提供可靠的检测工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

APOBApolipoprotein B ELISA kit; Fragment ELISA kit
缩写：

Apo-B100
Uniprot No.：

P17165
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

78 ng/mL-5000 ng/mL
灵敏度：

19.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	90-102
1:200	Average %	92
	Range %	87-96
1:400	Average %	104
	Range %	98-108
1:800	Average %	85
	Range %	80-89

回收率：

The recovery of rabbit Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-102
EDTA plasma (n=4)	87	82-92

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究进展：

Apo-B100是由APOB基因编码的蛋白质，是低密度脂蛋白（LDL）的主要成分之一。最新研究表明，Apo-B100在冠心病的发病中扮演了重要的角色。研究人员发现，Apo-B100水平升高与冠心病的风险增加有关。此外，一些研究还表明，调节Apo-B100的水平可以改善血脂和血糖代谢，从而降低心血管疾病的风险。
功能：

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
亚细胞定位：

Cytoplasm. Secreted. Lipid droplet.
组织特异性：

Detected in liver (at protein level). Highly expressed in liver. Detected at lower levels in jejunum, duodenum and kidney.
数据库链接：

STRING: 9986.ENSOCUP00000004631

UniGene: Ocu.1828