Rabbit apolipoprotein A1 (Apo-A1) ELISA Kit

Rabbit apolipoprotein A1 (Apo-A1) ELISA Kit

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中文名称：

兔载脂蛋白A1(Apo-A1)酶联免疫试剂盒
货号：

CSB-E09804Rb
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO兔载脂蛋白A1（Apo-A1）酶联免疫检测试剂盒（货号：CSB-E09804Rb），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的Apo-A1含量。Apo-A1作为高密度脂蛋白（HDL）的核心组分，在胆固醇逆向转运过程中发挥关键作用，其水平变化与脂质代谢调控、动脉粥样硬化等病理过程密切相关。试剂盒通过预包被特异性抗体实现高灵敏度检测，线性范围覆盖6.25至400 μg/mL，能够满足不同浓度样本的精确分析需求，实验全程可在4.5小时内完成。该试剂盒严格验证了抗体的种属特异性，确保对兔源样本检测的可靠性，适用于心血管疾病机制研究、药物干预效果评估或营养代谢相关动物模型中的生物标志物分析。研究人员可通过该工具动态监测实验动物在疾病发展或治疗过程中Apo-A1的表达变化，为探索脂蛋白代谢调控网络及开发相关干预策略提供数据支持。
别名：

APOA1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit
缩写：

APOA1
Uniprot No.：

P09809
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 μg/mL-400 μg/mL
灵敏度：

1.56 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	99
	Range %	91-105
1:4	Average %	89
	Range %	80-96
1:8	Average %	94
	Range %	85-99

回收率：

The recovery of rabbit Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	98	89-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

µg/ml	OD1	OD2	Average	Corrected
400	2.748	2.712	2.730	2.645
200	2.234	2.222	2.228	2.143
100	1.517	1.446	1.482	1.397
50	0.875	0.839	0.857	0.772
25	0.433	0.424	0.429	0.344
12.5	0.212	0.206	0.209	0.124
6.25	0.141	0.138	0.140	0.055
0	0.087	0.083	0.085

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

CETP ANTIGENIC PEPTIDE AND FUSION PROTEIN AND THEIR COMPOSITION AND APPLICATIONS Hwang J.et al,United States Patent,2016

功能：

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
基因功能参考文献：
1. Local HDAdApoAI vascular gene therapy may promote rapid regression of small-to-moderate-sized atherosclerotic lesions. PMID: 27932352
2. These differentially expressed proteins associated with key mechanisms involved in atherosclerosis and signaling mechanisms related with vitamin E. PMID: 23796494
3. This study showed that apoA-I exerted protective effects against fatty liver disease in rabbits induced by a high-fat diet, possibly through its antioxidant actions. PMID: 23174676
4. The molar ratio ApoE/ApoA-I is negatively correlated with the enzyme activity, and positively correlated with increases in the intima-media thickness of common carotid wall and cardiac dysfunction signs. PMID: 22562159
亚细胞定位：

Secreted.
蛋白家族：

Apolipoprotein A1/A4/E family
组织特异性：

Major protein of plasma HDL, also found in chylomicrons.
数据库链接：

KEGG: ocu:100009253

STRING: 9986.ENSOCUP00000009241

UniGene: Ocu.1800

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.