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  4. Rabbit Tumor necrosis factor α,TNFα ELISA Kit

Rabbit Tumor necrosis factor α,TNFα ELISA Kit

文献引用 (36) 评价 (2) 说明书下载
  • 中文名称：
    兔子肿瘤坏死因子α(TNF-α)酶联免疫试剂盒
  • 货号：
    CSB-E06998Rb
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO专用于兔源样本中肿瘤坏死因子α（TNF-α）定量检测的酶联免疫试剂盒（货号：CSB-E06998Rb），采用双抗体夹心法原理，可精准检测血清、血浆、组织匀浆及细胞裂解液等多种样本中的TNF-α浓度，检测灵敏度达78 pg/mL，线性范围为78-5000 pg/mL。TNF-α作为介导炎症反应和免疫调节的核心细胞因子，其水平变化与感染、自身免疫性疾病及肿瘤微环境调控等研究密切相关。本试剂盒通过高特异性抗体对实现目标蛋白的捕获与信号放大，适用于兔模型在病原体感染、炎症机制解析、药物干预效果评估等基础科研领域的研究需求，为探索TNF-α在免疫应答中的动态变化提供可靠工具，实验全程操作便捷，数据重复性优异，满足体外实验的标准化要求。
  • 别名：
    TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
  • 缩写：
    TNF
  • Uniprot No.：
  • 种属：
    Oryctolagus cuniculus (Rabbit)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TNFα in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 86  
    Range % 80-92  
    1:2 Average % 95  
    Range % 91-105  
    1:4 Average % 98  
    Range % 92-102  
    1:8 Average % 94  
    Range % 86-98  
  • 回收率：
    The recovery of rabbit TNFα spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 87-99  
    EDTA plasma (n=4) 92 87-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    5000 2.905 2.899 2.902 2.789  
    2500 2.366 2.356 2.361 2.248  
    1250 1.639 1.604 1.622 1.509  
    625 0.971 0.939 0.955 0.842  
    312 0.488 0.456 0.472 0.359  
    156 0.275 0.259 0.267 0.154  
    78 0.179 0.171 0.175 0.062  
    0 0.116 0.110 0.113 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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产品评价

平均分:
5.0分 - 2 个评价

样品类型：血浆（抗凝剂） EDTA

样品信息：其他 兔

稀释比：没有稀释

产品评价： 我用CSB-E06998Rb检测模型组术前，术中30min-2h，术后24h血浆，四参数模型拟合出来的R2为0.99868（详见图片），标准曲线符合预期。0-2h的TNF-a水平符合升高趋势，经过文献查询，该模型需要6h才能检测出大量升高的TNF-a炎症因子水平，后续会测量6h及以上的炎症因子水平。课题组师兄推荐，有兔子种属，且价格实惠，测试结果准确。

By 陈老师

样品类型：血浆（抗凝剂） EDTA

样品信息：其他 兔

稀释比：没有稀释

产品评价： 我用CSB-E06998Rb测模型组术前，术中30min-2h，术后24h血浆，标准曲线用四参数模型拟合出来的R2为0.99783（详见图片），标准曲线做了两列，符合预期且OD值在0至3.6范围内，且得到的数据符合预期趋势。该试剂盒测试稳定，均能显色。

By 陈老师

相关产品

TNF Proteins

靶点详情

  • 最新研究进展：
    肿瘤坏死因子（TNF）是一种细胞因子，在免疫调节、炎症和细胞凋亡等生物学过程中发挥重要作用。近年来的研究表明，TNF与各种疾病的发病机制有关，例如炎症性疾病、肿瘤、自身免疫性疾病等。一些新的抗TNF治疗药物也被开发出来，已经用于治疗类风湿性关节炎、克罗恩病等疾病，并获得了一定的疗效。
  • 功能：
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
  • 基因功能参考文献：
    1. TUNEL staining was positively correlated with TNF-a protein expression. Our findings suggest that apoptosis can be induced in the vocal fold epithelium after 120min of modal intensity phonation. In contrast, shorter durations of vibration exposure do not result in apoptosis signaling. PMID: 27577014
    2. Inflammatory factors such as TNF-alpha can stimulate MMP-2/9 activity in corneal epithelium cells. This may be a potential manipulating mechanism of MMP expression in the pathogenesis of corneal diseases. PMID: 26125840
    3. The JNK pathway plays an important role in mechanical ventilation-stimulated TNF-alpha expression in alveolar macrophages, but the injury-stimulated IL-8 expression may be regulated by other signaling pathways. PMID: 24070709
    4. The TNFalpha-evoked Cl- current. PMID: 24084720
    5. IL-1beta and TNF-alpha expression increases significantly during acute lung injury. Ambroxol combined with low-dose heparin inhibits teh release of IL-1beta and TNF-alpha. PMID: 21845882
    6. Hypercapnia increased expression of TNFa and decreased expression of NFKB in acute lung injury models. PMID: 21158126
    7. In the early stages of myocardial ischemia, bone marrow stem cells are mobilized and home to ischemic myocardium with a concomitant increase in expression of cytokines VEGF and TNFalpha. PMID: 20624659
    8. Ammonium perchlorate can increase gene expressions of types I, III collagens, TGF-beta(1) and TNF-alpha in lung of rabbits. PMID: 20937628
    9. TNFalpha may either be directly or indirectly involved in vascular damage following an embolic stroke. Moreover, TNFalpha may mediate some of the detrimental effects of tPA on the vascular compartment PMID: 17673188

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  • 亚细胞定位：
    Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
  • 蛋白家族：
    Tumor necrosis factor family
  • 数据库链接：

    KEGG: ocu:100009088

    STRING: 9986.ENSOCUP00000005787

    UniGene: Ocu.1971

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |