Rabbit Pulmonary surfactant-associated protein B(SFTPB) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

兔肺表面活性物质相关蛋白B(SFTPB)酶联免疫试剂盒（CSB-EL021173RB）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SFTPB含量。试剂盒检测范围为1.87 ng/mL-120 ng/mL，该产品适用于呼吸生理学研究，可探究SFTPB在肺损伤修复、感染性肺炎或遗传性肺泡蛋白沉积症等动物模型中的动态变化，也可用于评估肺表面活性物质代谢调控机制或药物干预效果。支持血清、血浆直接检测及肺组织等匀浆样本处理，满足不同实验设计的科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

SFTPB ELISA Kit; SFTP3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; 6 kDa protein ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
缩写：

SFTPB
Uniprot No.：

P15285
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.87 ng/mL-120 ng/mL
灵敏度：

0.46 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SFTPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	95
	Range %	91-99
1:4	Average %	94
	Range %	89-98
1:8	Average %	89
	Range %	86-96

回收率：

The recovery of rabbit SFTPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-98
EDTA plasma (n=4)	88	82-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
亚细胞定位：

Secreted, extracellular space, surface film.
数据库链接：

KEGG: ocu:100009194

STRING: 9986.ENSOCUP00000025658

UniGene: Ocu.1963