Rabbit Osteocalcin/bone gla protein,OT/BGP ELISA Kit

Rabbit Osteocalcin/bone gla protein,OT/BGP ELISA Kit

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中文名称：

兔子骨钙素/骨谷氨酸蛋白(OT/BGP)酶联免疫试剂盒
货号：

CSB-E14060Rb
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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本试剂盒（CSB-E14060Rb）采用夹心法原理定量检测兔来源样本（血清、血浆、组织匀浆、细胞裂解液）中的骨钙素/骨谷氨酸蛋白（OT/BGP），该蛋白是骨基质中由成骨细胞分泌的重要非胶原蛋白，通过γ-羧化谷氨酸残基参与钙离子结合，其表达水平可反映骨形成活性，常用于骨代谢调控机制、骨质疏松症模型评估及骨骼相关药物开发等研究。试剂盒在1.25-80 ng/mL范围内呈现良好线性，最低检测限为0.08 ng/mL，标准品采用冻干粉形式，配套含保护剂的检测缓冲液可降低基质干扰。实验需自备酶标仪（450nm）、移液器等基础设备，提供预包被特异性抗体的96孔板、生物素标记检测抗体、链霉亲和素-HRP等核心组分，适用于体外分析骨组织工程材料对成骨细胞功能的影响、疾病模型中骨代谢动态监测、以及骨相关生物标志物的基础研究。建议实验前通过预实验优化样本稀释比例，组织样本推荐使用含蛋白酶抑制剂的裂解液制备匀浆，所有组分需按说明书规范保存和使用。
别名：

BGLAP ELISA Kit; Osteocalcin ELISA Kit; Bone Gla protein ELISA Kit; BGP ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit
缩写：

BGLAP
Uniprot No.：

P39056
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

1.25 ng/mL-80 ng/mL
灵敏度：

0.31 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit OT/BGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	95
	Range %	89-99
1:4	Average %	94
	Range %	90-90
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit OT/BGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	90-94
EDTA plasma (n=4)	92	87-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
80	2.780	2.701	2.741	2.641
40	2.124	2.056	2.090	1.990
20	1.266	1.234	1.250	1.150
10	0.667	0.621	0.644	0.544
5	0.353	0.335	0.344	0.244
2.5	0.262	0.249	0.256	0.156
1.25	0.219	0.208	0.214	0.114
0	0.102	0.098	0.100

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Uniform-sized insulin-loaded PLGA microspheres for improved early-stage peri-implant bone regeneration Wang X, et al,Drug Delivery,2019

功能：

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
亚细胞定位：

Secreted.
蛋白家族：

Osteocalcin/matrix Gla protein family
数据库链接：

STRING: 9986.ENSOCUP00000020318

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.