Rabbit Myeloperoxidase (MPO) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO兔源髓过氧化物酶（MPO）定量检测试剂盒（货号：CSB-E13689Rb），基于竞争法原理开发，适用于血清、血浆、细胞培养上清及组织匀浆样本中MPO蛋白的定量分析。MPO是一种主要存在于中性粒细胞中的血红素蛋白酶，在先天免疫防御中通过催化次氯酸生成发挥杀菌作用，其表达水平与炎症反应、氧化应激等病理过程密切相关。本试剂盒检测灵敏度为1.25 ng/mL，线性检测范围覆盖1.25-20 ng/mL，实验体系包含预包被96孔板、系列浓度标准品、生物素标记检测抗体及显色底物，通过竞争性结合反应实现目标蛋白的精准定量，操作全程可在4小时内完成。该试剂盒适用于基础科研中炎症机制研究、免疫相关疾病动物模型评估、药物干预效果分析等场景，例如通过检测实验动物血清或组织样本中的MPO水平，定量评估中性粒细胞浸润程度及相关疾病的病理进程，为心血管疾病、神经退行性疾病等领域的机制探索提供可靠数据支持。
缩写：

MPO
Uniprot No.：

G1SR65
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

1.25 ng/mL-20 ng/mL
灵敏度：

1.25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-103
1:2	Average %	87
	Range %	84-90
1:4	Average %	95
	Range %	89-99
1:8	Average %	101
	Range %	98-105

回收率：

The recovery of rabbit MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	93	89-97