Rabbit Matrix Metalloproteinase 13,MMP-13 ELISA Kit

Rabbit Matrix Metalloproteinase 13,MMP-13 ELISA Kit

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中文名称：

兔子基质金属蛋白酶13(MMP-13)酶联免疫试剂盒
货号：

CSB-E14220Rb
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO针对兔基质金属蛋白酶13（MMP-13）定量检测的酶联免疫试剂盒（货号：CSB-E14220Rb），采用双抗体夹心法原理，可精准检测血清、血浆及组织匀浆样本中MMP-13的浓度，检测范围为93.75-6000 pg/mL。MMP-13作为基质金属蛋白酶家族成员，在细胞外基质降解、关节炎病理进程、组织修复及肿瘤转移调控等生物学过程中发挥重要作用，其表达水平检测对相关疾病机制研究具有重要科研价值。本试剂盒适用于体外实验研究，可满足关节炎模型构建、骨代谢研究、肿瘤微环境分析等科研场景中MMP-13的动态监测需求，实验操作便捷，标准曲线线性稳定，配套提供预包被抗体及显色系统，确保实验数据的可靠性和重复性。
别名：

MMP13Collagenase 3 ELISA Kit; EC 3.4.24.- ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP-13 ELISA Kit
缩写：

MMP13
Uniprot No.：

O62806
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

93.75 pg/mL-6000 pg/mL
灵敏度：

23.44 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	105
	Range %	97-108
1:4	Average %	90
	Range %	85-95
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of rabbit MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	83-95
EDTA plasma (n=4)	98	90-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
6000	2.554	2.444	2.499	2.405
3000	1.937	1.976	1.957	1.863
1500	1.409	1.422	1.416	1.322
750	0.815	0.789	0.802	0.708
375	0.422	0.434	0.428	0.334
187.5	0.302	0.289	0.296	0.202
93.75	0.207	0.198	0.203	0.109
0	0.093	0.095	0.094

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

In Vitro and In vivo approaches to evaluate Uncaria tomentosa bark extract loaded FDOFs on Osteoarthritis models JN Sowjanya - 2023 - researchsquare.com,researchsquare,2023
Chondroprotective and antiarthritic effects of daphnetin used in vitro and in vivo osteoarthritis models Xiaohan Zhang, et al,Life Sciences,2019
Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone Li Y. et al,Osteoarthritis Cartilage,2015

功能：

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
基因功能参考文献：
1. These results suggest that PEP-1-SIRT2 promotes matrix metalloproteinases-induced dedifferentiation via ERK signaling in articular chondrocytes. PMID: 27697532
2. the total content and active forms of matrix metalloproteinases (MMPs)-1, -3, and -13 was measured to assess the potential role of the mechanical environment in regulation of matrix turnover by ligament fibroblasts. PMID: 23879595
3. chitosan-pDNA nanoparticles encoding shRNA targeting MMP-3 and -13 had great potential in silencing the dedifferentiation-related genes for regenerating prolonged and endurable cartilage. PMID: 23520014
4. Leptin can induce MMP-13 and have a synergistic induction effect on NO with TNF-alpha in rabbit articular chondrocytes in vitro. PMID: 19893248
亚细胞定位：

Secreted, extracellular space, extracellular matrix. Secreted.
蛋白家族：

Peptidase M10A family
数据库链接：

KEGG: ocu:100008685

STRING: 9986.ENSOCUP00000002145

UniGene: Ocu.2263

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.