Rabbit Macrophage Inflammatory Protein-1α,MIP-1α ELISA kit

产品详情

产品描述：

兔子巨噬细胞炎性蛋白1α(MIP-1α/CCL3)酶联免疫试剂盒（CSB-E04625Rb）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MIP1α/CCL3含量。MIP1α/CCL3，即巨噬细胞炎症蛋白1-a/CCL3，是CC亚家族的趋化因子，主要由单核细胞、巨噬细胞等免疫细胞产生。它通过结合细胞表面受体CCR1、CCR3和CCR5，诱导白细胞趋化，促进T细胞进入炎症区域，并在肉芽肿、哮喘等自身免疫性疾病中发挥关键作用。试剂盒检测范围为7.8 pg/mL-500 pg/mL，科研人员可将其应用于炎症机制研究、疾病模型中的趋化因子动态监测、免疫调节药物筛选等方向，为探索MIP - 1α在先天免疫应答、感染性疾病或自身免疫病中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

MIP-1 alpha,Macrophage Inflammatory Protein-1 alpha
缩写：

MIP1α/CCL3
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

7.8 pg/mL-500 pg/mL
灵敏度：

1.95 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	105
	Range %	93-109
1:2	Average %	110
	Range %	97-114
1:4	Average %	88
	Range %	83-93
1:8	Average %	95
	Range %	85-98

回收率：

The recovery of rabbit MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	95-109
EDTA plasma (n=4)	101	91-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.