Rabbit Leptin,LEP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO针对兔源瘦素(LEP)定量检测的竞争法酶联免疫试剂盒（货号：CSB-E06971Rb），适用于分析血清、血浆及组织匀浆样本中的LEP含量。瘦素是由脂肪细胞分泌的关键代谢调节激素，通过作用于下丘脑调控能量平衡与食欲，其水平异常与肥胖、胰岛素抵抗等代谢性疾病密切相关。CUSABIO试剂盒采用预包被抗体的微孔板结构，通过生物素标记物与样本中LEP竞争结合位点实现定量检测，灵敏度达15.6 pg/mL，线性范围为15.6-1000 pg/mL，可精准检测微量样本（需50μL）中的目标蛋白浓度。该产品适用于科研领域中对兔模型能量代谢机制、脂肪组织功能、肥胖相关疾病机制的研究，尤其适用于评估脂肪细胞分泌活性或药物干预后LEP的动态变化。试剂盒包含标准品、检测抗体、显色底物等全套组分，提供96孔板规格，支持手动或半自动酶标仪（450nm波长）操作，4℃保存有效期达6个月，-20℃长期保存可维持性能稳定。
缩写：

LEP
Uniprot No.：

B0KZL0
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	91-99
1:200	Average %	93
	Range %	88-97
1:400	Average %	85
	Range %	80-89
1:800	Average %	90
	Range %	84-96

回收率：

The recovery of rabbit LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-105
EDTA plasma (n=4)	87	83-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.