Rabbit Krebs yon den Lundgen-6 (KL-6) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO针对兔源Ⅱ型肺泡细胞表面抗原（KL-6）检测开发的酶联免疫试剂盒（货号：CSB-E14112Rb），采用竞争法原理实现定量分析，适用于血清、血浆及组织匀浆样本的检测。KL-6是由Ⅱ型肺泡上皮细胞分泌的粘蛋白样糖蛋白，在肺组织损伤修复及纤维化进程中发挥重要作用，其水平变化常被用于研究肺部炎症、间质性肺疾病等病理机制。试剂盒检测范围覆盖50 IU/mL至800 IU/mL，通过预包被特异性抗体的酶标板捕获样本中的目标抗原，结合HRP标记检测抗体及TMB显色系统实现精准定量分析。该试剂盒包含标准品、生物素化抗体、酶结合物及样本稀释液等核心组分，支持科研人员在体外实验中定量评估肺损伤模型、药物干预研究或疾病生物标志物探索中的KL-6动态变化，为呼吸系统疾病的基础研究提供灵敏可靠的工具。
缩写：

KL-6
Uniprot No.：
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

50 IU/mL-800 IU/mL
灵敏度：

25 IU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit KL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	94
	Range %	89-99
1:4	Average %	92
	Range %	87-96
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of rabbit KL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	89	87-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.