Your Good Partner in Biology Research

Rabbit Interleukin 2,IL-2 ELISA Kit

  • 中文名称:
    兔子白介素2(IL-2)酶联免疫试剂盒
  • 货号:
    CSB-E08745Rb
  • 规格:
    96T/48T
  • 价格:
    ¥3800/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO专用于科研领域的兔子白介素2(IL-2)酶联免疫检测试剂盒(货号:CSB-E08745Rb),采用夹心法原理实现定量分析,可精准检测血清或血浆样本中31.25-2000 pg/mL浓度范围的IL-2蛋白。IL-2是由活化T淋巴细胞分泌的关键细胞因子,在免疫调节中发挥重要作用,通过促进T细胞增殖、分化及调节免疫应答参与机体抗感染和免疫稳态维持。该试剂盒包含预包被捕获抗体的酶标板、系列浓度标准品、生物素标记检测抗体及链霉亲和素-HRP系统,通过双抗体夹心法实现高特异性结合,操作流程包含样本孵育、洗板、显色及OD值测定,实验全程约需4.5小时。其宽泛的检测范围可满足不同浓度样本需求,适用于动物模型研究、免疫机制探索、炎症相关疾病机制分析或疫苗效力评估等科研场景,为探究IL-2在免疫系统中的调控作用提供可靠工具。试剂盒经优化验证,具备良好的批间稳定性和灵敏度,数据重复性满足科研实验要求。
  • 别名:
    IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
  • 缩写:
  • Uniprot No.:
  • 种属:
    Oryctolagus cuniculus (Rabbit)
  • 样本类型:
    serum, plasma
  • 检测范围:
    31.25 pg/mL-2000 pg/mL
  • 灵敏度:
    7.8 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %87
    Range %82-93
    1:2Average %95
    Range %91-100
    1:4Average %86
    Range %80-95
    1:8Average %93
    Range %82-98
  • 回收率:
    The recovery of rabbit IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9280-98
    EDTA plasma (n=4)9384-100
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.412 2.320 2.366 2.181
    10002.174 2.081 2.128 1.943
    5001.882 1.743 1.813 1.628
    2501.521 1.482 1.502 1.317
    1251.048 1.044 1.046 0.861
    62.50.688 0.654 0.671 0.486
    31.250.331 0.308 0.320 0.135
    00.191 0.179 0.185  
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • 基因功能参考文献:
    1. IL-2 activates the STAT3 pathway, protects the lens epithelium cells and reduce the damage caused by inflammation reactions. PMID: 18201527
  • 亚细胞定位:
    Secreted.
  • 蛋白家族:
    IL-2 family
  • 数据库链接: