Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rabbit Interleukin 1β,IL-1β ELISA Kit

Rabbit Interleukin 1β,IL-1β ELISA Kit

文献引用 (14) 说明书下载
  • 中文名称：
    兔子白介素1β (IL-1β)酶联免疫试剂盒
  • 货号：
    CSB-E06900Rb
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO定量检测兔源白介素1β（IL-1β）的酶联免疫试剂盒（货号CSB-E06900Rb），采用双抗体夹心法原理，可精准检测血清、血浆及组织匀浆样本中7.8-500 pg/mL浓度范围的IL-1β蛋白。IL-1β作为促炎细胞因子，在免疫应答、炎症反应及组织损伤修复中发挥核心调控作用，其动态表达水平与感染性疾病、自身免疫病及神经退行性病变等研究密切相关。该试剂盒配套标准品与高特异性抗体，操作流程简便，适用于体外实验中对动物模型（如关节炎、脓毒症等疾病模型）的炎症机制研究、药物干预效果评估或免疫调节通路分析。试剂盒兼容多种生物样本类型，为科研工作者在基础医学、药理学及免疫学领域提供灵敏稳定的检测工具，支持细胞因子网络调控的深入探索。
  • 别名：
    IL1BInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit; Lymphocyte proliferation-potentiating factor ELISA kit
  • 缩写：
    IL1B
  • Uniprot No.：
  • 种属：
    Oryctolagus cuniculus (Rabbit)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %98
    Range %85-105
    1:2Average %95
    Range %85-103
    1:4Average %94
    Range %82-100
    1:8Average %95
    Range %85-105
  • 回收率：
    The recovery of rabbit IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9682-104
    EDTA plasma (n=4)9585-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.852 2.713 2.783 2.674
    2502.235 2.245 2.240 2.131
    1251.424 1.392 1.408 1.299
    62.50.753 0.784 0.769 0.660
    31.20.447 0.433 0.440 0.331
    15.60.266 0.282 0.274 0.165
    7.80.202 0.195 0.199 0.090
    00.111 0.106 0.109 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

显示更多
收起更多

产品评价

相关产品

IL1B Proteins

靶点详情

  • 功能：
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
  • 基因功能参考文献：
    1. Mild heat shock increased the production of inflammatory cytokines, IL-1beta and IL-6 in rabbit cornea cells. PMID: 23330884
    2. IL-1beta and TNF-alpha expression increases significantly during acute lung injury. Ambroxol combined with low-dose heparin inhibits teh release of IL-1beta and TNF-alpha. PMID: 21845882
    3. IL-1beta induced a significant reduction in the relative intrinsic activity of GLUT5 and in this decrease are involved NO signal pathways; blockage of D-fructose intestinal uptake by IL-1beta may play an essential role in the pathophysiology of septic shock. PMID: 20803526
    4. The inhibitory effect of IL-1beta on D-galactose absorption across mucosal side of enterocyte could be mediated by the activation of several kinases and nuclear factor kappa B. PMID: 22759965
    5. Glucosamine hydrochloride treatment can can partially decrease the expression levels of IL-1 beta and increase the expression levels of TGF-beta 1, which delays the development of osteoarthritis. PMID: 20369526
    6. In this study evidence is provided that exogenous PGF2alpha differentially modulates luteal expression of IL1B transcripts depending on luteal stage. PMID: 20427144
    7. results revealed that a transient episode of raised-intensity phonation causes a significant increase in vocal fold inflammatory mRNA expression - IL-1beta,COX-2, and TGFbeta1 PMID: 20879195

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
  • 蛋白家族：
    IL-1 family
  • 数据库链接：

    KEGG: ocu:100008990

    STRING: 9986.ENSOCUP00000021869

    UniGene: Ocu.1993

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |