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中文名称:兔白介素17(IL-17)酶联免疫试剂盒
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货号:CSB-E08058Rb
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:兔白介素17(IL-17)酶联免疫试剂盒(CSB-E08058Rb)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的IL17含量。IL-17 是重要的细胞因子,在免疫应答和炎症反应中起关键作用。它与自身免疫性疾病、感染性疾病等多种疾病相关。研究机制方面,主要聚焦其信号通路、与其他细胞因子相互作用等,以探索通过干预 IL-17 治疗相关疾病的方法。试剂盒检测范围为31.25 pg/mL-2000 pg/mL,适用于炎症模型构建、免疫应答机制研究、药物干预效果评价等科研场景,例如通过分析感染或自身免疫疾病模型中血清IL - 17水平变化,或探究特定组织内细胞因子表达特征,为免疫相关基础研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
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缩写:IL-17
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Uniprot No.:
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种属:Oryctolagus cuniculus (Rabbit)
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样本类型:serum, plasma, tissue homogenates
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检测范围:31.25 pg/mL-2000 pg/mL
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灵敏度:7.81 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Immunology
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 89 Range % 81-96 1:2 Average % 94 Range % 85-105 1:4 Average % 95 Range % 87-102 1:8 Average % 91 Range % 83-99 -
回收率:
The recovery of rabbit IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 85-100 EDTA plasma (n=4) 95 84-106 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 2000 2.860 2.712 2.786 2.670 1000 2.248 2.212 2.230 2.114 500 1.392 1.387 1.390 1.274 250 0.754 0.729 0.742 0.626 125 0.456 0.432 0.444 0.328 62.5 0.275 0.267 0.271 0.155 31.25 0.213 0.199 0.206 0.090 0 0.119 0.112 0.116 -
数据处理:
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货期:3-5 working days
引用文献
- Prokaryotic Expression of Eimeria magna SAG10 and SAG11 Genes and the Preliminary Evaluation of the Effect of the Recombinant Protein on Immune Protection in Rabbits J Pu,International journal of molecular sciences,2022
- Pathogenesis of intradermal staphylococcal infections: rabbit experimental approach to natural Staphylococcus aureus skin infections A Mu帽oz-Silvestre,The American Journal of Pathology,2020
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