Rabbit Interleukin 10,IL-10 ELISA Kit

Rabbit Interleukin 10,IL-10 ELISA Kit

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中文名称：

兔子白介素10(IL-10)酶联免疫试剂盒
货号：

CSB-E06897Rb
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO试剂盒采用双抗体夹心酶联免疫吸附法，专为定量检测兔源样本中白介素10（IL-10）蛋白水平而设计。IL-10作为关键的抗炎细胞因子，主要由Th2细胞、调节性T细胞和巨噬细胞分泌，在调控免疫应答平衡、抑制过度炎症反应中发挥重要作用，是研究免疫调节机制及炎症相关疾病的重要靶标。试剂盒检测范围覆盖31.25-2000 pg/mL，适用于血清、血浆及组织匀浆样本的检测，无需复杂样本前处理。通过预包被特异性捕获抗体和标记检测抗体的协同作用，配合显色反应实现精准定量，实验全程可在4.5小时内完成。该产品广泛应用于免疫学研究领域，特别适用于评估炎症性疾病模型、感染免疫应答或免疫调节药物干预实验中IL-10的动态变化，为探索免疫调控机制提供可靠工具。试剂盒包含标准品、预包被96孔板及检测所需全套试剂，实验结果可通过酶标仪在450nm波长下读取，支持多批次实验的稳定性和重复性验证。
别名：

IL10 ELISA Kit; Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
缩写：

IL10
Uniprot No.：

Q9TSJ4
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-98
1:2	Average %	96
	Range %	91-105
1:4	Average %	97
	Range %	92-110
1:8	Average %	93
	Range %	86-100

回收率：

The recovery of rabbit IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	89	80-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.627	2.549	2.588	2.461
1000	1.954	1.799	1.877	1.750
500	1.004	0.987	0.996	0.869
250	0.599	0.554	0.577	0.450
125	0.369	0.361	0.365	0.238
62.5	0.274	0.264	0.269	0.142
31.25	0.177	0.171	0.174	0.047
0	0.132	0.122	0.127

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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In Vivo Pathogenicity of Methicillin-Susceptible Staphylococcus aureus Strains Carrying Panton–Valentine Leukocidin Gene F Yildirim,Life,2022
Prokaryotic Expression of Eimeria magna SAG10 and SAG11 Genes and the Preliminary Evaluation of the Effect of the Recombinant Protein on Immune Protection in Rabbits J Pu,International journal of molecular sciences,2022
Immunization with Virus-Like Particle Vaccine Protects Rabbits against Hepatitis E-3 Virus Infection HJ Go,Viruses,2022
Mesenchymal stem cells significantly improved treatment effects of Linezolid on severe pneumonia in a rabbit model Kong D, et al,Bioscience Reports,2019
Evaluation of the protective effects of a nanogel-based vaccine against rabbit hepatitis E virus Byung-JooPark, et al,Vaccine,2019
Poly(Lactic Acid) Blends with Poly(Trimethylene Carbonate) as Biodegradable Medical Adhesive Material Zhang S.et al,Int. J. Mol. Sci,2017
Clinical Features of Chronic Trypanosomosis in Rabbits Experimentally Infected with the Malaysian Isolate ofTrypanosoma evansi Yasameen et al,IOSR-JAVS,2015

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功能：

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
亚细胞定位：

Secreted.
蛋白家族：

IL-10 family
数据库链接：

STRING: 9986.ENSOCUP00000019534

UniGene: Ocu.1796

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.