Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rabbit Interferon γ,IFN-γ ELISA Kit

Rabbit Interferon γ,IFN-γ ELISA Kit

文献引用 (8) 说明书下载
  • 中文名称：
    兔子γ干扰素(IFN-γ)酶联免疫试剂盒
  • 货号：
    CSB-E06925Rb
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO试剂盒采用双抗体夹心法原理，专为定量检测兔血清、血浆及组织匀浆样本中的γ干扰素（IFN-γ）设计。IFN-γ是由T淋巴细胞和NK细胞分泌的关键细胞因子，在固有免疫与适应性免疫应答中发挥核心作用，其表达水平与病毒感染、自身免疫性疾病及肿瘤免疫调控等过程密切相关。试剂盒检测灵敏度为62.5 pg/mL，线性范围覆盖62.5-4000 pg/mL，可精准检测样本中IFN-γ的浓度梯度变化。该产品适用于多种科研场景，包括但不限于动物免疫机制研究、疫苗效力评估实验、感染性疾病模型中的免疫应答分析以及炎症相关信号通路研究。实验操作中需准备标准品和样本进行梯度稀释，通过酶标仪在450nm波长下读取吸光值并绘制标准曲线。试剂盒包含预包被特异性抗体的96孔板、检测抗体、标准品、显色底物等完整组分，经严格验证具备良好的批间重复性，可为免疫学、兽医学及基础医学研究提供可靠的数据支持。
  • 别名：
    IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
  • 缩写：
    IFNG
  • Uniprot No.：
  • 种属：
    Oryctolagus cuniculus (Rabbit)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %85-105
    1:2Average %94
    Range %85-105
    1:4Average %96
    Range %92-100
    1:8Average %95
    Range %83-105
  • 回收率：
    The recovery of rabbit IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9585-100
    EDTA plasma (n=4)9585-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.862 2.713 2.788 2.673
    20002.214 2.229 2.222 2.107
    10001.373 1.397 1.385 1.270
    5000.755 0.731 0.743 0.628
    2500.446 0.432 0.439 0.324
    1250.263 0.275 0.269 0.154
    62.50.202 0.194 0.198 0.083
    00.117 0.113 0.115 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

显示更多
收起更多

产品评价

相关产品

IFNG Proteins

靶点详情

  • 功能：
    Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Type II (or gamma) interferon family
  • 组织特异性：
    Released primarily from activated T lymphocytes.
  • 数据库链接：

    KEGG: ocu:100008602

    STRING: 9986.ENSOCUP00000021866

    UniGene: Ocu.2149

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |