Rabbit Insulin-like growth factor 1,IGF-1 ELISA Kit

Rabbit Insulin-like growth factor 1,IGF-1 ELISA Kit

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中文名称：

兔子胰岛素样生长因子1(IGF-1)酶联免疫试剂盒
货号：

CSB-E06994Rb
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO专为科研领域设计的兔子胰岛素样生长因子1（IGF-1）酶联免疫检测试剂盒（货号：CSB-E06994Rb），采用双抗体夹心法定量分析血清、血浆及组织匀浆样本中的IGF-1含量。IGF-1是一种重要的多肽生长因子，参与调节细胞增殖、分化及代谢过程，在生长发育、组织修复及能量稳态等研究中具有关键作用。试剂盒检测灵敏度达0.156 ng/mL，线性范围覆盖0.156-10 ng/mL，可精准检测微量样本中的靶蛋白浓度，适用于兔类动物模型的代谢研究、生长发育评估及与IGF-1相关的疾病机制探索，如糖尿病、肌肉萎缩等病理状态分析。实验操作兼容常规酶标仪检测，无需特殊设备，96孔板规格满足高通量研究需求。本产品严格进行批次质控，提供标准品及预包被板条，确保实验数据的可靠性和重复性，为分子生物学、生理学及兽医学领域的科研工作者提供高效工具。
别名：

IGF1 ELISA Kit; IGF-1Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
缩写：

IGF1
Uniprot No.：

Q95222
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	80-100
1:2	Average %	100
	Range %	91-105
1:4	Average %	98
	Range %	92-115
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of rabbit IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	90	85-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.715	2.725	2.720	2.605
5	2.035	2.002	2.019	1.904
2.5	0.989	1.078	1.034	0.919
1.25	0.591	0.602	0.597	0.482
0.625	0.342	0.352	0.347	0.232
0.312	0.201	0.212	0.207	0.092
0.156	0.175	0.185	0.180	0.065
0	0.111	0.119	0.115

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

The effects of IGF-1 and IGFBP-2 treatments on the atherosclerosis in the aorta and the coronary arteries of the high cholesterol diet-fed rabbits W Wang,International immunopharmacology,2023
Hair Follicle Development of Rex Rabbits Is Regulated Seasonally by Wnt10b/β-Catenin, TGFβ-BMP, IGF1, and EGF Signaling Pathways G Liu,Animals,2023
Construction of engineered corpus cavernosum with primary mesenchymal stem cells in vitro Xie X.et al,Sci Rep,2017

功能：

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.
基因功能参考文献：
1. Data suggest caloric restriction modifies response of ovarian granulosa cells to leptin on cell proliferation/apoptosis, MAP kinase signaling, and release of hormones (estradiol and IGFI). The caloric restriction studies were conducted in rabbits; then, isolated rabbit granulosa cells were exposed to recombinant human leptin. (IGF1 = insulin-like growth factor I) PMID: 27894742
2. Intraplacental gene therapy with Ad-IGF-1 corrects naturally occurring rabbit model of intrauterine growth restriction. PMID: 25738403
3. Our studies demonstrate that the active SVCT2 is expressed in IVD cells and that the expression of this transporter is regulated by growth factors IGF-1 and dexamethasone. PMID: 23415019
4. increased endogenous IGF1 and IGF2 expression by the blastocyst compensates for the loss of systemic insulin and IGF. PMID: 22465205
5. IGF1 showed a significantly higher capacity to form the posterior mesoderm and primitive streak (stage 2 and 3) than blastocysts cultured without growth factors PMID: 21827825
6. Overall, IGF-1 promoted atherosclerosis by affecting endothelial function and aging. PMID: 21219916
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亚细胞定位：

Secreted.
蛋白家族：

Insulin family
数据库链接：

KEGG: ocu:100008668

STRING: 9986.ENSOCUP00000021389

UniGene: Ocu.2125

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.