Rabbit Fibronectin(FN1) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO兔纤连蛋白（FN1）酶联免疫检测试剂盒（货号：CSB-EL008759RB），专为科研领域设计，采用竞争法检测原理，通过特异性抗体与目标蛋白结合实现定量分析。纤连蛋白是细胞外基质的关键成分，参与细胞黏附、迁移及组织修复等生物学过程，其表达水平变化与胚胎发育、炎症反应及纤维化疾病等研究密切相关。试剂盒检测范围覆盖1.25 ng/mL至20 ng/mL，支持血清、血浆及组织匀浆样本的高灵敏度检测，最低可准确检测至1.25 ng/mL。实验操作基于预包被微孔板，配套标准品和生物素标记检测系统，适用于实验室对FN1蛋白表达水平的动态监测，可广泛用于心血管疾病模型、纤维化机制研究、肿瘤微环境分析等基础科研领域，为探索细胞外基质调控机制提供可靠工具。试剂盒包含完整实验组分，优化了孵育条件和显色体系，确保检测稳定性和重复性，满足体外样本中FN1蛋白的定量需求。
别名：

FN1Fibronectin ELISA Kit; FN ELISA Kit; Fragment ELISA Kit
缩写：

FN1
Uniprot No.：

Q28749
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.25 ng/mL-20 ng/mL
灵敏度：

1.25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit FN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	92-98
1:200	Average %	103
	Range %	98-108
1:400	Average %	98
	Range %	94-102
1:800	Average %	92
	Range %	87-97

回收率：

The recovery of rabbit FN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	96-101
EDTA plasma (n=4)	89	82-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
亚细胞定位：

Secreted, extracellular space, extracellular matrix.
组织特异性：

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
数据库链接：

STRING: 9986.ENSOCUP00000008718

UniGene: Ocu.1875