Rabbit Endothelin 1,ET-1 ELISA Kit

Rabbit Endothelin 1,ET-1 ELISA Kit

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中文名称：

兔子内皮素1(ET-1)酶联免疫试剂盒
货号：

CSB-E06951Rb
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO专用于科研领域的兔子内皮素1（ET-1）酶联免疫检测试剂盒（货号：CSB-E06951Rb），可定量检测血清、血浆及组织匀浆样本中的ET-1水平。ET-1作为一种强效血管收缩肽，在心血管调节、炎症反应和细胞增殖等生理病理过程中发挥重要作用。试剂盒采用双抗体夹心法原理，检测灵敏度达3.12 pg/mL，线性检测范围为6.25-400 pg/mL，操作时仅需100 μL样本，约4小时即可完成检测流程。试剂盒组分包含预包被捕获抗体的96孔板、检测抗体及标准品，保存于2-8℃环境下有效期长达12个月，开封后建议按需分装避免反复冻融。该产品适用于心血管疾病机制研究、内皮功能评估、药物干预效果分析等基础科研领域，支持在动物模型或离体实验中定量分析ET-1表达动态，为血管生物学、病理生理学等研究提供稳定可靠的数据支持。
别名：

EDN1Endothelin-1 ELISA Kit; ET-1 ELISA Kit; Preproendothelin-1 ELISA Kit; PPET1) [Cleaved into: Big endothelin-1] ELISA Kit
缩写：

EDN1
Uniprot No.：

P29560
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	100
	Range %	91-105
1:4	Average %	96
	Range %	92-104
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	98	90-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.630	2.456	2.543	2.458
200	2.111	2.076	2.094	2.009
100	1.459	1.443	1.451	1.366
50	0.816	0.769	0.793	0.708
25	0.415	0.389	0.402	0.317
12.5	0.218	0.203	0.211	0.126
6.25	0.147	0.149	0.148	0.063
0	0.088	0.082	0.085

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

CT perfusion imaging of cerebral microcirculatory changes following subarachnoid hemorrhage in rabbits: Specific role of endothelin-1 receptor antagonist QuanyangWu.et al,Brain Research Bulletin,2018
Blast Wave Exposure to the Extremities Causes Endothelial Activation and Damage. Spear AM.et al,Shock.,2015

功能：

Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells.
基因功能参考文献：
1. Study shows that cerebrovascular muscle cells can express endothelin-1 (ET-1). The production of ET-1 in cerebrovascular muscle cells stimulated by oxyhemoglobin occurs partially through the NF-[kappa]B signal pathway. PMID: 27391329
2. Plasma ET-1 levels of rabbits increased significantly in fluorinated groups compared with those in the control group. PMID: 27941335
3. Dynamic monitoring and comparison of plasma levels of ET, CGRP, NO, and MDA as well as SOD activity revealed that appropriate intervention of these factors may reduce reperfusion injury PMID: 26125755
4. In a saline lavage-induced lung injury model, both circulatory and pulmonary ET-1 levels increased. PMID: 24735960
5. Data suggest elevated levels of endothelin-1, as exhibited in cardiovascular diseases, facilitate development of ventricular arrhythmia by steepening action potential duration restitution and by increasing beat-to-beat variability of repolarization. PMID: 24084217
6. High levels of ET-1 are closely associated with BBB disruption. ET-1 may play an important role in the pathogenesis of secondary brain injury after ICH. PMID: 24034085
7. After subarachnoid hemorrhage, the contractile response to ET-1 was enhanced, and the ET(A) receptor expression was upregulated in the basilar artery. PMID: 21952110
8. After acute pulmonary thromboembolism, thrombolytic and anti-inflammatory treatment could decrease acute lung injury induced by ET-1 and NF-kappaB activation. [endothelin-1; NFKB] PMID: 16207427
9. The increase in myocardial distensibility induced by endothelin-1 is absent in heart failure and is dependent on nitric oxide and prostaglandin release. PMID: 17552879
10. Endothelin-1 enhances nuclear Ca2+ transients in atrial myocytes through Ins(1,4,5)P3-dependent Ca2+ release from perinuclear Ca2+ stores PMID: 18089647
11. The enhancement of gap junction intercellular communication is activated by endothelin-1 via modulating the expression of connexin43, and plays an important role in the pathogenesis of cerebral vasospasm PMID: 18691452
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亚细胞定位：

Secreted.
蛋白家族：

Endothelin/sarafotoxin family
数据库链接：

KEGG: ocu:100009270

STRING: 9986.ENSOCUP00000011526

UniGene: Ocu.5758

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.