Rabbit Cortisol ELISA Kit

产品详情

产品描述：

CUSABIO兔源皮质醇（Cortisol）酶联免疫检测试剂盒（货号：CSB-E06956Rb），适用于定量检测血清、血浆及组织匀浆样本中的皮质醇浓度。皮质醇是肾上腺分泌的关键糖皮质激素，广泛参与机体应激反应、代谢调节及免疫抑制等生理过程，其水平变化常作为应激状态、内分泌功能及相关疾病研究的生物标志物。CUSABIO试剂盒采用竞争法检测原理，检测范围为62.5-1000 pmol/mL，可精准覆盖生物样本中皮质醇的常见浓度区间。实验操作基于预包被板进行，通过标准品与样本中皮质醇竞争结合特异性抗体，结合显色反应实现定量分析，流程简便高效。该产品适用于科研领域，如动物应激模型研究、药物对肾上腺功能影响的评估，或生理病理状态下皮质醇动态变化的分析，为神经内分泌学、药理学及基础医学研究提供可靠工具。试剂盒提供完整组分，包括标准品、检测抗体及优化缓冲体系，支持重复性检测需求。
别名：

N/A
缩写：

Cortisol
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pmol/mL-1000 pmol/mL
灵敏度：

62.5 pmol/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	81-97
1:2	Average %	89
	Range %	82-98
1:4	Average %	90
	Range %	85-100
1:8	Average %	95
	Range %	89-105

回收率：

The recovery of rabbit Cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-99
EDTA plasma (n=4)	95	89-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.