Rabbit Coagulation factor VII(F7) ELISA kit

中文名称：

兔凝血因子VII(F7)酶联免疫试剂盒
货号：

CSB-EL007930RB
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO定量检测兔血清、血浆或组织匀浆中凝血因子VII（F7）含量的竞争法ELISA试剂盒，检测灵敏度达3.9 ng/mL，线性检测范围为3.9-250 ng/mL，适用于科研领域对凝血机制、出血性疾病模型或药物干预效果的研究。凝血因子VII是外源性凝血途径的关键启动因子，通过与组织因子结合激活下游凝血级联反应，其表达水平与血栓形成、术后出血等生理病理过程密切相关。该试剂盒采用预包被抗原的酶标板，通过样本中的F7与生物素标记F7竞争结合特异性抗体的原理，结合链霉亲和素-HRP显色系统实现定量检测，显色强度与样本中F7浓度呈负相关。配套提供标准品、检测抗体及终止液等完整组分，支持研究者高效完成心血管疾病模型、肝脏功能评估或抗凝药物开发等实验中的F7水平监测，操作全程无需特殊设备，3小时内即可获得稳定检测结果。
别名：

F7Coagulation factor VII ELISA Kit; EC 3.4.21.21 ELISA Kit; Serum prothrombin conversion accelerator) [Cleaved into: Factor VII light chain; Factor VII heavy chain] ELISA Kit
缩写：

F7
Uniprot No.：

P98139
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.9 ng/mL-250 ng/mL
灵敏度：

1 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Blood Coagulation
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	85
	Range %	80-90
1:40	Average %	104
	Range %	100-107
1:80	Average %	87
	Range %	83-90
1:160	Average %	102
	Range %	98-106

回收率：

The recovery of rabbit F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-93
EDTA plasma (n=4)	110	107-113

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
250	0.134	0.130	0.132
125	0.219	0.221	0.220
62.5	0.378	0.381	0.380
31.2	0.565	0.581	0.573
15.6	0.868	0.858	0.863
7.8	1.209	1.236	1.223
3.9	1.598	1.569	1.584
0	2.399	2.401	2.400

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
基因功能参考文献：
1. Results suggest that inhibition of FVIIa with small-molecule active-site inhibitors represents a promising antithrombotic approach. PMID: 20589312
亚细胞定位：

Secreted.
蛋白家族：

Peptidase S1 family
组织特异性：

Plasma.
数据库链接：

KEGG: ocu:100009399

STRING: 9986.ENSOCUP00000024648

UniGene: Ocu.2617

Rabbit Coagulation factor VII(F7) ELISA kit

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