Rabbit C-Reactive Protein,CRP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO兔子C反应蛋白（CRP）酶联免疫检测试剂盒（货号：CSB-E06847Rb），基于双抗体夹心法原理设计，可对血清、血浆样本中的CRP进行精准定量分析。C反应蛋白是一种由肝脏合成的急性期反应蛋白，在炎症、感染或组织损伤等生理过程中显著升高，其浓度变化常作为炎症反应的关键生物标志物，广泛用于动物模型中的免疫调控机制研究、炎症相关疾病模型评估及药物干预效果分析。该试剂盒检测范围为7.8 pg/mL至2000 pg/mL，采用预包被特异性抗体的微孔板，通过标准品梯度稀释实现高灵敏度检测，能够稳定识别低丰度样本中的CRP水平。其操作流程兼容常规ELISA实验步骤，适用于科研领域中对兔类动物炎症相关研究的样本检测，例如实验性关节炎模型、感染性疾病模型或代谢综合征研究中血清/血浆CRP的动态监测，为探索炎症通路机制及潜在治疗靶点提供可靠数据支持。本产品仅限科学研究使用，不涉及临床应用。
别名：

CRP ELISA kit; PTX1C-reactive protein ELISA kit
缩写：

CRP
Uniprot No.：

P02742
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma
检测范围：

7.8 pg/mL-2000 pg/mL
灵敏度：

2 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	96
	Range %	90-102
1:2000	Average %	91
	Range %	82-96
1:4000	Average %	90
	Range %	85-98
1:8000	Average %	94
	Range %	83-98

回收率：

The recovery of rabbit CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-104
EDTA plasma (n=4)	91	85-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
基因功能参考文献：
1. Rabbit CRP acute-phase system binds phosphocholine and phosphoethanolamine. PMID: 23600950
2. IL-6 augments mechanical strain-induced CRP synthesis in vessels via the stretch-activated channel-NF-kappaB pathway. PMID: 23257175
3. CRP antagonism of eNOS is mediated by coupling of FcgammaRI to FcgammaRIIB by Src kinase and activation of inositol 5'-phosphatase 1, and consistent with this mechanism, both FcgammaRI and FcgammaRIIB are required for CRP to blunt endothelial repair in vivo. PMID: 21940940
4. Metformin attenuated the progression of aortic atherosclerosis via down-regulation of serum C-reactive protein. PMID: 20108078
5. During abdominal aortic aneurysm formation, increased serum hsCRP levels derive from aneurysmal arteries with degenerating elastic lamina. PMID: 19502992
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亚细胞定位：

Secreted.
蛋白家族：

Pentraxin family
组织特异性：

Found in plasma.
数据库链接：

KEGG: ocu:100009091

STRING: 9986.ENSOCUP00000004059

UniGene: Ocu.1848