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  4. Rabbit Bone morphogenetic protein 2(BMP2) ELISA kit

Rabbit Bone morphogenetic protein 2(BMP2) ELISA kit

说明书下载
  • 中文名称：
    兔子骨形成蛋白(BMP2)酶联免疫试剂盒
  • 货号：
    CSB-EL002736RB
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO针对兔源骨形成蛋白2(BMP2)研发的酶联免疫检测试剂盒（货号：CSB-EL002736RB），适用于血清、血浆及组织匀浆样本中BMP2的定量分析。BMP2属于TGF-β超家族成员，在骨骼与软骨形成中发挥核心调控作用，同时参与细胞分化、增殖及凋亡等生物学过程。试剂盒采用双抗体夹心法原理，通过预包被的捕获抗体与样本中BMP2结合，经生物素标记的检测抗体二次识别后，借助链霉亲和素-HRP催化显色反应实现定量检测，灵敏度达1.56 pg/mL，线性范围为1.56-100 pg/mL，可在4小时内完成实验流程。该产品适用于骨骼发育机制、组织再生修复、骨代谢相关疾病模型等基础研究，也可用于评估骨生长因子类药物在动物实验中的生物效应，实验操作兼容常规酶标仪检测系统，配套提供标准品、预混试剂及详细操作指南，为科研人员提供稳定可靠的蛋白定量工具。
  • 别名：
    BMP2 ELISA Kit; BMP-2Bone morphogenetic protein 2 ELISA Kit; BMP-2 ELISA Kit
  • 缩写：
    BMP2
  • Uniprot No.：
  • 种属：
    Oryctolagus cuniculus (Rabbit)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 pg/mL-100 pg/mL
  • 灵敏度：
    0.39 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit BMP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:2 Average % 87
    Range % 80-94
    1:4 Average % 90
    Range % 85-99
    1:8 Average % 95
    Range % 85-103
    1:10 Average % 95
    Range % 84-102
  • 回收率：
    The recovery of rabbit BMP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 93 82-100
    EDTA plasma (n=4) 97 88-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    100 2.136 2.177 2.157 2.043
    50 1.612 1.628 1.620 1.506
    25 1.221 1.239 1.230 1.116
    12.5 0.852 0.865 0.859 0.745
    6.25 0.530 0.516 0.523 0.409
    3.12 0.351 0.365 0.358 0.244
    1.56 0.239 0.246 0.243 0.129
    0 0.112 0.115 0.114 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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BMP2 Proteins

靶点详情

  • 功能：
    Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis. Induces cartilage and bone formation. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation. Stimulates also the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation.
  • 基因功能参考文献：
    1. The transfecting capability of a BMP-2 specific vector is examined and supports the idea that BMP-2 might diminish osseointegration and implant fixation. PMID: 21762501
    2. While BMP2 expression begins at (pregastrulation) stage 1 in the hypoblast, BMP4 expression commences--distinctly delayed compared to the mouse--diffusely at (pregastrulation) stage 2; from stage 3 onwards. PMID: 21881976
    3. Maxillary sinus floor elevation using BMP-2 gene-modified bone marrow stromal cells and TCP in rabbits PMID: 21584647
    4. Data show that BMP-2, BMP-4, and BMP-7, noggin, and chordin were colocalized in rimming osteoblasts, osteoclasts, and chondrocytes. PMID: 21099737
    5. BMP2 is not suitable to regenerate osteochondral lesions completely PMID: 19185914

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family
  • 数据库链接：

    KEGG: ocu:100009349

    STRING: 9986.ENSOCUP00000010036

    UniGene: Ocu.2192

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |