Rabbit Bone Alkaline Phosphatase (BALP) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专用于科研领域的兔子骨碱性磷酸酶（BALP）定量检测试剂盒（货号：CSB-E15042Rb），可精准检测血清、血浆样本中的BALP含量。BALP是成骨细胞特异性分泌的酶类标志物，在骨基质矿化过程中发挥关键作用，其活性水平与骨形成速率密切相关，是研究骨代谢调控、骨发育异常及骨修复机制的重要生物指标。CUSABIO试剂盒采用双抗体夹心法原理，通过预包被特异性抗体实现靶标捕获，检测灵敏度达0.156 mIU/mL，线性范围为0.156-10 mIU/mL，可覆盖生理及病理状态下BALP的浓度变化。试剂盒适用于骨代谢相关动物模型研究，如骨质疏松症机制探索、骨折愈合进程监测、骨再生药物疗效评估等科研场景，为体外定量分析提供稳定可靠的数据支持。实验操作全程无需特殊设备，兼容常规酶标仪读取结果，配套提供标准品及优化缓冲体系，确保实验重复性与数据准确性。
缩写：

BALP
Uniprot No.：

G1SLU6
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma
检测范围：

0.156 mIU/mL-10 mIU/mL
灵敏度：

0.039 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	88-101
1:2	Average %	88
	Range %	81-92
1:4	Average %	89
	Range %	85-94
1:8	Average %	98
	Range %	90-101

回收率：

The recovery of rabbit BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	95-108
EDTA plasma (n=4)	99	91-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.