Rabbit Aggrecan core protein(ACAN) ELISA kit

Rabbit Aggrecan core protein(ACAN) ELISA kit

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中文名称：

兔子蛋白聚糖(ACAN)酶联免疫试剂盒
货号：

CSB-E12831Rb
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO试剂盒采用双抗体夹心法原理，可定量检测兔血清、血浆及组织匀浆中的蛋白聚糖(ACAN)含量，适用于软骨代谢机制、关节疾病模型等基础研究领域。ACAN作为细胞外基质核心成分，在维持软骨结构完整性和机械性能中起关键作用，其表达水平变化常与退行性关节病变、组织修复等过程相关。试剂盒检测灵敏度达0.625 ng/mL，线性范围覆盖40 ng/mL，通过优化包被抗体与标记抗体组合，确保对兔源样本的特异性识别。实验全程可在4-6小时内完成，支持96孔板高通量检测，提供预包被板条、标准品及全套检测试剂，用户仅需自备酶标仪即可开展实验。该产品适用于体外评估软骨细胞分泌功能、病理模型中的基质代谢动态监测，或生物材料对软骨再生的影响等科研场景，实验结果需结合其他生物学方法综合分析。本产品仅供科学研究使用。
别名：

ACAN ELISA Kit; AGC1 ELISA Kit; Aggrecan core protein ELISA Kit; Cartilage-specific proteoglycan core protein ELISA Kit; CSPCP ELISA Kit; Fragment ELISA Kit
缩写：

ACAN
Uniprot No.：

Q28670
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ACAN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	90-103
1:2	Average %	89
	Range %	82-94
1:4	Average %	90
	Range %	82-94
1:8	Average %	94
	Range %	87-98

回收率：

The recovery of rabbit ACAN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-94
EDTA plasma (n=4)	103	93-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.020	2.121	2.071	1.944
20	1.902	1.846	1.874	1.747
10	1.570	1.524	1.547	1.420
5	1.238	1.200	1.219	1.092
2.5	0.955	0.934	0.945	0.818
1.25	0.532	0.545	0.539	0.412
0.625	0.315	0.309	0.312	0.185
0	0.128	0.126	0.127

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Pilot-scale expression, purification, and bioactivity of recombinant human TGF-β3 from Escherichia coli MengZhou, et al,European Journal of Pharmaceutical Sciences,2018

功能：

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.
蛋白家族：

Aggrecan/versican proteoglycan family
数据库链接：

STRING: 9986.ENSOCUP00000024918

UniGene: Ocu.2050

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.