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中文名称:植物1,5-二磷酸核酮糖羧化酶/加氧酶(RuBisCO)酶联免疫试剂盒
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货号:CSB-E16686Pl
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:本产品是专为植物样本中1,5-二磷酸核酮糖羧化酶/加氧酶(RuBisCO)定量检测设计的酶联免疫试剂盒,该酶是植物光合作用卡尔文循环中的核心催化酶,参与CO₂固定和光呼吸调控,其表达水平与植物光合效率、逆境响应及代谢调控密切相关。试剂盒采用竞争法检测原理,适用于叶片、茎等植物组织样本检测,检测范围覆盖3.12-800 μg/mL,通过预包被特异性抗体与样本中RuBisCO竞争结合酶标抗原,实现目标蛋白的定量分析。该试剂盒提供96孔板规格,包含标准品、检测抗体、显色底物等完整组分,操作流程约需3.5小时完成。适用于植物生理学研究中光合作用机制解析、环境胁迫(如高温、干旱)对酶活性影响评估、转基因植物代谢通路改造效果验证等科研场景,为植物抗逆性改良、作物产量提升等研究提供可靠工具。
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缩写:RuBisCO
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种属:Plant
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样本类型:plant tissues
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检测范围:3.12 μg/mL-800 μg/mL
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灵敏度:3.12 μg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Cell Biology
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测定原理:quantitative
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测定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<10% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<20% Three samples of known concentration were tested in twenty assays to assess. -
回收率:
The recovery of plant RuBisCO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range leaf tissue (n=5) 95 89-103 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. μg/ml OD1 OD2 Average 800 0.047 0.050 0.049 200 0.261 0.268 0.265 50 0.467 0.473 0.470 12.5 0.670 0.682 0.676 3.12 1.139 1.143 1.141 0 1.861 1.916 1.889 -
数据处理:
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货期:3-5 working days
引用文献
- Foliar Application of Carbon Quantum Dots Photosynthesis Enhancer for Enhancing the Growth and Development of Green Mustard (Brassica Juncea) in Indoor Hydroponics N Izati Fadzil, R Nulit, M Jusoh, S Abdul Rashid, TL Tan,SSRN,/
- Low-level expression of induced defences and increased compensation in Eupatorium adenophorum relative to its native congeneric plant to mechanical wounding and insect herbivory RF Wang,Journal of Plant Ecology,2023
- Opposite Growth Responses of Alexandrium minutum and Alexandrium catenella to Photoperiods and Temperatures P Li,Plants,2021
- High antioxidant capability interacts with respiration to mediate two Alexandrium species growth exploitation of photoperiods and light intensities WangHui, et al,Harmful Algae,2019
靶点详情
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最新研究进展:RuBisCO是一种催化光合作用的酶,在植物和一些细菌中广泛存在。最近的研究发现,RuBisCO还可以在大气中吸收和固定二氧化碳,并且可以通过调节RuBisCO的活性和结构来提高植物对气候变化的适应能力。
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