Pig prolactin (PRL) ELISA Kit

Pig prolactin (PRL) ELISA Kit

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中文名称：

猪催乳素(PRL)酶联免疫试剂盒
货号：

CSB-E15801p
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

猪催乳素(PRL)酶联免疫试剂盒（CSB-E15801p）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的PRL含量。PRL即泌乳素，是腺垂体分泌的多肽激素。其分泌受下丘脑调节，参与乳腺发育、泌乳启动与维持等生理过程。近年研究发现它还与肿瘤生长、转移相关，在肿瘤微环境中影响免疫细胞功能，成为潜在抗癌治疗靶点，研究其机制有重要意义。试剂盒检测范围为0.78 ng/ml-50 ng/ml.，本试剂盒通过高特异性抗体对目标蛋白进行捕获与检测，适用于实验室环境下针对猪血清或血浆样本的PRL水平分析，可服务于动物生殖生理机制研究、泌乳相关调控通路探索以及农业养殖中繁殖效率评估等科研方向。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
缩写：

PRL
Uniprot No.：

P01238
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma
检测范围：

0.78 ng/ml-50 ng/ml.
灵敏度：

0.195 ng/ml
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	99
	Range %	87-95
1:2	Average %	94
	Range %	89-97
1:4	Average %	99
	Range %	94-102
1:8	Average %	95
	Range %	91-99

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

2.677

2.713

2.695

2.495

1.886

1.901

1.894

1.694

12.5

1.365

1.412

1.389

1.189

6.25

1.000

1.032

1.016

0.816

3.12

0.785

0.792

0.789

0.589

1.56

0.546

0.554

0.550

0.350

0.78

0.346

0.324

0.335

0.135

0.199

0.200

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Impacts du seigle hybride dans l'alimentation des truies en gestation et en début de lactation sur les performances de reproduction et le microbiote intestinal des truies et leurs porcelets L Mazroua,Thesis&Dissertation,2024
Valine supplementation during late pregnancy in gilts increases colostral protein synthesis through stimulating mTOR signaling pathway in mammary cells Long Che, et al,Amino Acids,2019
Effects of maternal alpha-ketoglutarate supplementation during lactation on the performance of lactating sows and suckling piglets Jiang Q, et al,Archives of Animal Nutrition,2019
Effect of optimizing dietary valine-to-lysine ratio in late gestation or lactation on biochemical indices and performance of lactating primiparous sows KaiguoGao,Animal Feed Science and Technology,2019

功能：

Prolactin acts primarily on the mammary gland by promoting lactation.
基因功能参考文献：
1. Prolactin and, to a lesser extent progesterone, which increase in early pregnancy, enhance basal and glucose-stimulated insulin secretion in part by increasing glucokinase activity and amplifying cAMP levels. PMID: 14559722
2. iv infusion of prolactin primarily caused coronary, mesenteric, renal, and iliac vasoconstriction PMID: 17463060
3. despite a lack of clear relationship between PRL/C499T polymorphism in 5' UTR of the PRL gene and plasma prolactin cioncentration, gene polymorphism impact on reproductiove performance cannot be excluded PMID: 19675522
亚细胞定位：

Secreted.
蛋白家族：

Somatotropin/prolactin family
数据库链接：

KEGG: ssc:396965

STRING: 9823.ENSSSCP00000001152

UniGene: Ssc.8204

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.